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11691112001 Roche

Human Genomic DNA

from human blood (buffy coat)

Synonym: dna, human, homo sapiens dna

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Properties

Related Categories Genomic DNA Amplification, Molecular Biology, PCR/Amplification, from Blood
Quality Level   100
form   solution
packaging   pkg of 100 μg
mfr. no.   Roche
concentration   0.2 mg/mL
shipped in   wet ice
storage temp.   2-8°C

Description

General description

High molecular weight (>50kb) genomic DNA isolated from human blood (buffy coat) by the method of Sambrook et al..

Application

This DNA is suitable for Southern hybridization analysis, genomic library construction, and the amplification of large DNA targets by the Expand System. It has been used to assess the quality or integrity of DNA sample using qPCR or real time (RT) PCR, and as a control during DNA sequencing.
The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.

Quality

Molecular weight: The preparation is electrophoretically separated on a 0.5% agarose gel and the gel is stained with ethidium bromide. The molecular weight of the purified genomic DNA is greater than 50kb.
Function test: The preparation is used as template in a PCR with the Expand PCR System and appropriate primers from the human tPA Control Primer Set. Amplification products up to 27kb long are obtained.
Absence of contaminating organisms: The serum used for this preparation was tested for HBs antigen and the presence of antibodies to HIV-1, HIV-2, HCV. All tests were negative.

Physical form

Solution in 10 mM Tris HCl, 1 mM EDTA, pH 8.0

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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