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11699105001 Roche

PCR Buffer Without MgCl2, 10x concentrated

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Properties

Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
Quality Level   100
form   solution
packaging   pkg of 3 × 1 mL
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

General description

The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.

Application

The PCR buffer without MgCl2 is used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).
The buffer is ten-fold concentrated and should be used in combination with the MgCl2 Stock Solution.

Physical form

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Other Notes

For general laboratory use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

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PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
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