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11699113001 Roche

MgCl2 Stock Solution

Synonym: magnesium chloride solution



Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
Quality Level   100
form   solution
packaging   pkg of 3 × 1 mL
mfr. no.   Roche
concentration   25 mM
shipped in   dry ice
storage temp.   −20°C


General description

The MgCl2 Stock Solution is used for the individual adjustment of the Mg2+-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl2. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and the yield of the PCR reaction.


The combination of PCR Buffer without MgCl2 and the MgCl2 Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl2 Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).

Biochem/physiol Actions

Magnesium (Mg2+) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR). It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.

Physical form

The MgCl2 Stock Solution contains 25mM MgCl2 in sterile water.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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