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11699121001 Roche

PCR Buffer Set

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Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
Quality Level   100
packaging   pkg of 2 × 2 mL
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

General description

The kit-provided PCR buffer without MgCl2, in combination with the kit-provided MgCl2 stock solution, are used for the individual adjustment of the Mg2+ concentration. In most PCR applications, a concentration of 1.5 mM MgCl2 will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg2+ concentration for high PCR specificity and amplicon yield.

Application

For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase.

Packaging

1 set containing 2 components

1 set containing 3 components

Quality

Function test: Each lot of buffer is tested in PCR.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

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MgCl2 Stock Solution 25 mM    
PCR Buffer without Mg2+ 10x concentrated    
Alkaline Phosphatase Substrate, CSPD    
Enhancer, Emerald II    
Assay Buffer    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

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PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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