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11719394001 Roche

Immunoprecipitation Kit (Protein A)

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Properties

usage   sufficient for 20 reactions
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

Application

For the immunoprecipitation of proteins from cellular extracts with Protein A Agarose.

General description

Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 5 components.

Preparation Note

Working solution: Lysis buffer/wash buffer 1
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.

Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

Kit component only

Description

Product #

Add to Cart

Core Buffer    
NaCl    
Detergent Mix    
cOmplete Protease Inhibitor Cocktail Tablets (5)    
Protein A Agarose ready-to-use    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting

Cell lysis and sample preparation 1. Wash cells/tissue at least twice with ice-cold PBS to remove any remaining serum proteins from the culture medium. For one immunoprecipitation reaction a sample v...
Keywords: Adsorption, Buffers, Cell culture, Cell disruption, Centrifugation, Degradations, Detergents, Electrophoresis, Enzyme activity, Gel electrophoresis, Homogenization, Immunoprecipitation, PAGE, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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