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11787896001 Roche

mRNA Capture Kit

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Properties

Related Categories DNA & RNA Purification, Molecular Biology, RNA Purification, Roche NAP Kits
Quality Level   100
mfr. no.   Roche
packaging   kit of for 192 isolations

Description

General description

mRNA Capture Kit; Instructions For Use

Affinity purification is a versatile and highly specific technique for the purification of virtually all classes of biomolecules utilizing differences in biological activities of chemical structures. The high selectivity of this technique results in good purification and high recovery. Often a concentrating effect is reached which enables large volumes to be conveniently processed.

The mRNA Capture Kit enables preparation of highly purified poly (A)+ RNA, which may be used directly for qualitative or quantitative RT-PCR.

Application

The mRNA Capture Kit isolates highly purified poly (A)+ RNA and immobilizes it in a PCR tube. The immobilized mRNA is suitable for:

• Qualitative and quantitative RT-PCR
• cDNA-AFLP

mRNA Capture Kit has been used for the extraction of messenger RNA from monocytes and B cell subsets.

Features and Benefits

Flexible - Use kit from small to high numbers of samples and for semi-automatic procedures. Use kit from small to high numbers of samples and for semi-automatic procedures.
Efficient - Minimize sample loss, handling time, and contamination risk because mRNA isolation and RT-PCR can be performed in the same tube.
Reliable - Produce excellent RT-PCR templates, even from small amounts of starting material, or material containing low-abundance mRNAs.

Components

• Lysis Buffer
• Oligo (dT)20, biotin-labeled, 20x concentrated
• Water, double-distilled, PCR Grade
• Wash Buffer
• Streptavidin-Coated PCR Tubes
• Caps for PCR Tubes

Quality

The kit is function tested and tested for the absence of RNase according to the current quality control procedures.

Preparation Note

A lysis step releases RNA from cultured cells or tissues The poly (A)+ tail of mRNA in the lysate hybridizes to a biotin-labeled oligo (dT)20. The hybrid is then immobilized on a streptavidin-coated PCR tube, followed by washing steps to remove unbound contaminants. The oligo (dT) part of this immobilized hybrid also serves as an RT-PCR primer for reverse transcriptase. The entire process (including RT-PCR) takes place in a single tube. The capture process requires approximately 30 minutes.

Other Notes

Application of the Roche mRNA Capture Kit in cDNA-AFLP Article (PDF, 499 KB)

Isolation of poly-A(+)-RNA from Single Cells Using the mRNA Capture Kit Article (PDF, 829.57 KB)

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

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