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11810740001 Roche

In Situ Cell Proliferation Kit, FLUOS

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Properties

Quality Level   100
usage   sufficient for ≤100 tests
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Immunofluorescence assay for the detection of 5-Bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.

Specificity

The anti-BrdU antibody specifically binds to BrdU. It shows no cross-reactivity with any endogenous cellular component, such as thymidine or uridine. The antibody only binds to BrdU incorporated into DNA after denaturation/partial degradation of double-stranded DNA.

Application

In Situ Cell Proliferation Kit, FLUOS has been used in DNA synthesis analysis ((5-bromo-2′-deoxyuridine) BrdU assay) and immunofluorescence.

Features and Benefits

• Safe: No radioactive isotopes are used
• Easy to perform: Follows a standard immunohistochemistry protocol
• Specific: No crossreactivity with endogenous IgG
• Fast: Direct antibody conjugate, no secondary detection system required

Packaging

1 kit containing 3 components.

Preparation Note

Working solution: Preparation of the working solutions

BrdU labeling solution
For in vitro labeling, predilute 0.1 ml BrdU labeling reagent with 9.9 ml sterile culture medium (resulting concentration: 100 M BrdU).
For in vivo labeling, use the BrdU labeling reagent undiluted.

Anti-BrdUFLUOS antibody working solution
Dissolve lyophilizate in 1 ml double-dist. water. Immediately before use, dilute anti-BrdU-FLUOS stock solution 1:5 with incubation buffer.
Storage conditions (working solution): BrdU labeling solution
Stable for one month at 2 to 8 °C; for long-term storage in aliquots at -15 to -25 °C.
Anti-BrdUFLUOS antibody working solution
Working solution: cannot be stored.
The undiluted anti-BrdU-FLUOS stock solution: stable at 2 to 8 °C in the dark for 12 months.

Reconstitution

Fixative:
1. Add 113 mg glycine to 25 ml double-dist. water; adjust pH with NaOH or HCl to 2.0, if necessary.
2. Fill up to 30 ml.
3. Add 70 ml pure ethanol and mix thoroughly.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit component only

Description

Product #

Add to Cart

BrdU Labeling Reagent    
Anti-BrdU-FLUOS antibody 5x concentrated    
Incubation Buffer ready-to-use    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

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