EMAIL THIS PAGE TO A FRIEND
11814320001 Roche

Formamide

Synonym: Amide C1, Formamide, Formic amide

  • CAS Number 75-12-7

  • Linear Formula HCONH2

  • Molecular Weight 45.04

  •  Beilstein/REAXYS Number 505995

  •  MDL number MFCD00007941

  •  PubChem Substance ID 329749563

Purchase

Properties

Related Categories Acids and Bases, Biochemicals and Reagents, Denaturation More...
Quality Level   100
vapor density   1.55 (vs air)
vapor pressure   0.08 mmHg ( 20 °C)
  30 mmHg ( 129 °C)
assay   ≥99%
form   liquid
autoignition temp.   932 °F
mol wt   45.04
packaging   pkg of 500 mL
mfr. no.   Roche
color   colorless
refractive index   n20/D 1.447 (lit.)
bp   210 °C (lit.)
mp   2-3 °C (lit.)
density   1.134 g/mL at 25 °C (lit.)
absorption   ≤0.08 at 290 nm
shipped in   wet ice
storage temp.   2-8°C
SMILES string   NC=O
InChI   1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key   ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.

Application

Formamide has been used in:
in situ hybridization
• as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
• for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
• as a component of stop buffer used in cleavage and polyadenylation activity assays

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Quality

Contaminants: ≤5 ppm heavy metals (as Pb), ≤5 ppm Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?