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11836153001 Roche

cOmplete, Mini Protease Inhibitor Cocktail

Tablets provided in a glass vial

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Properties

Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzymes, Inhibitors, and Substrates, Protease Inhibitor Cocktails, Protease Inhibitors More...
form   tablet
packaging   vial of 25 tablets
mfr. no.   Roche
solubility   water: soluble
shipped in   wet ice
storage temp.   2-8°C

Description

General description

cOmplete, Mini Protease Inhibitor Tablets, inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains.
Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete, Mini Protease Inhibitor Tablets contain EDTA and both irreversible and reversible protease inhibitors.

Specificity

For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.

Application

cOmplete, Mini Protease Inhibitor Cocktail has been used as a
component of
• lysis buffer for lysing glioma tumor samples
• radioimmunoprecipitation (RIPA) protein extraction buffer for homogenization of heart tissues
• resuspension buffer for retinal ganglion cells homogenization

cOmplete, Mini, is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not  just during the initial purification steps.
cOmplete, Mini contains EDTA (1 tablet yields a  1 mM EDTA solution  in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal  Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
Alternatively, the product cOmplete, Mini EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is  present.
cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml) we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore comparable results are achieved with both product types.

Features and Benefits

Use cOmplete, Mini Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

• Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
• Complete protection: Instantly protect your proteins against a broad range of proteases.
• Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
• Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
• Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

Components

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Quality

Performance-tested with pancreas extract.

Physical form

Tablets in glass vials

Preparation Note

Working concentration: 1 tablet per 10 ml extraction solution
Working solution: Preparation of Working Solution

One cOmplete Mini Protease Inhibitor Tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (7 X conc.) can be prepared.

Stock solution (7 X conc.)

Dissolve one cOmplete Mini tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

cOmplete is a trademark of Roche

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Protein Stabilization Reagents from Roche

Naturally-occurring proteases and phosphatases can destroy proteins that you spent days isolating. With Roche’s experience in protein isolation, purification, and protein protection against degradati...
Keywords: Affinity chromatography, Cell disruption, Chromatography, Degradations, Immobilization, Phosphorylations, Purification, Tagged proteins

Protocols

cOmplete™, Mini Protocol

cOmplete Inhibition of Endoproteinase Asp-N cOmplete inhibits the metalloprotease endoproteinase Asp-N. cOmplete tablets for use in preparing subcellular fractions from intestine This has not been te...
Keywords: Buffers, Cell culture, Dialysis

Peer-Reviewed Papers
15

References

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