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11855476001 Roche

Titan One Tube RT-PCR System

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Properties

Related Categories Molecular Biology, PCR/Amplification, RT-PCR and RT-qPCR More...
usage   sufficient for 100 reactions
mfr. no.   Roche
parameter   42-60 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Titan One Tube RT-PCR System uses Reverse Transcriptase AMV for first strand cDNA synthesis and the Expand High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by PCR. The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
• Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.
• Is faster than conventional two-step RT-PCR.

Specificity

Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Application

• The one-tube reaction system will reverse transcribe RNA into cDNA with Reverse Transcriptase AMV, then amplify the cDNA with a thermal cycling reaction using the optimized enzyme blend from the Expand High Fidelity PCR System. Depending on the primers used for the RT-PCR techniques, a specific message or the entire population of transcripts can be amplified. It can be also used for:Determining the presence or absence of RNA templates
• Cloning of rare messages without the need to construct cDNA libraries
• Quantifying the levels of gene expression

Packaging

1 kit containing 4 components

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit component only

Description

Product #

Add to Cart

Reverse Transcriptase AMV and Expand High Fidelity Enzyme Mix in storage buffer    
RT-PCR Reaction Buffer 5x concentrated    
MgCl2 Stock Solution, 25 mM    
DTT Solution, 100 mM    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Titan One Tube RT-PCR System Protocol & Troubleshooting

Amount of poly(A) RNA template For best results, start with 1 ng - 100 ng poly(A)-RNA. Template preparation The required template RNA can be isolated using the mRNA Capture Kit or the mRNA Isolation ...
Keywords: Amplification, Gene expression, Nucleic acid denaturation, Obesity, Polymerase chain reaction, Respiratory

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Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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