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11888412001 Roche

PCR Nucleotide MixPLUS

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Properties

Related Categories 10 mM Solutions, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
form   solution
usage   sufficient for 500 reactions
packaging   pkg of 2 × 100 μL (10 mM each)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

The PCR Nucleotide MixPLUS can be added directly to amplification reactions.PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. They can be used to amplify even low amounts of template RNA and DNA.
Improve yield and performance
• Choose a convenient ready-to-use mix of all 4 nucleotides.
• Achieve the highest PCR and RT-PCR sensitivity.
• Insist on highest purity (>99%) nucleotides

General description

PCR Nucleotide MixPLUS is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, each at a concentration of 10 mM, and dUTP at a concentration of 30 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.
The incorporation of dUTP in place of dTTP allows the degradation of contaminating PCR products from former reactions with Uracil-DNA Glycosylase (UNG) to prevent carryover contamination from previous amplifications.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 set containing 4 ready-to-use mixes

Quality

Function tested in PCR to ensure specific DNA amplification. Decontamination with UNG is verified. No RNases or DNases are detectable according to the current Quality Control procedures.

Application

• This ready-to-use nucleotide mix is a premixed solution of the sodium salts of dATP, dGTP, and dCTP, each at a concentration of 10 mM, and dUTP at a concentration of 30 mM in water. This mix is optimized for use in all types of amplification reactions: PCR
• RT-PCR
• Prevention of carryover contamination
To allow decontamination of PCR or RT-PCR, dUTP in place of dTTP is incorporated into the PCR product. Subsequent reactions may then be treated with Uracil-DNA Glycosylase (UNG). Avoiding the need of re-opening the reaction vial, the vials are incubated at +20 °C, resulting in the degradation of potentially contaminating uracil-containing amplification products. During this step, template DNA and RNA remain unaffected, since normal DNA does not contain uracil, and RNA does not serve as a substrate for UNG. Before starting the actual thermocycling program, UNG is inactivated by incubation at +95 °C. Uracil -DNA Glycosylase, heat-labile is particularily useful, as it is fully inactivated already after incubation at +95 °C for 2 minutes. The natural enzyme from E. coli requires incubating the reaction mixture for 10 minutes at +95 °C. The shorter heat treatment substantially reduces the risk for loosing the template nucleic acid, which typically is present at low concentrations only. This is of particular importance, when performing RT-PCR. We therefore recommend the use of Uracil-DNA Glycosylase.
It has been used in LightCycler PCR assay to identify B. pertussis and B.parapertussis in nasopharyngeal swabs. It has also been used in quantitative PCR (qPCR) assay.

Kit component only

Description

Product #

Add to Cart

dATP, PCR Grade, sodium salt 10 mM    
dCTP, PCR Grade, sodium salt 10 mM    
dGTP, PCR Grade, sodium salt 10 mM    
dUTP, PCR Grade, sodium salt 10 mM    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles

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Peer-Reviewed Papers
15

References

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