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DATP-RO Roche

dATP

PCR Grade, sodium salt

Synonym: 2′-Deoxyadenosine 5′-triphosphate sodium salt solution, dATP

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Properties

Related Categories 100 mM Solutions, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
Quality Level   100
assay   <0.9% (dNDP)
  99% (dNTP)
form   solution
usage   sufficient for 125,000 standard reactions (03732681001)
  sufficient for 31,250 standard reactions (11969013001)
  sufficient for 6,250 standard reactions (11934511001)
packaging   pkg of 1,250 μL (11969013001 [100 mM])
  pkg of 4 × 1,250 μL (03732681001 [4x100 mM])
  pkg of 250 μL (11934511001 [100 mM])
mfr. no.   Roche
concentration   100 mM
shipped in   dry ice
storage temp.   −20°C
SMILES string   [Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3
InChI   1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1
InChI key   YJWCICGGRLOGEH-VWZUFWLJSA-M

Description

General description

dATP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Quality
Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

dATP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Biochem/physiol Actions

dATP is used in cells for synthesis of DNA by DNA polymerases.

Physical form

solution of dATP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
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References

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