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DTTP-RO Roche

dTTP

PCR Grade, sodium salt

Synonym: TTP, Thymidine 5′-triphosphate sodium salt solution

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Properties

Related Categories 100 mM Solutions, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
Quality Level   100
assay   <0.9% (dNDP)
  99% (dNTP)
form   solution
usage   sufficient for 125,000 standard reactions (03732711001)
  sufficient for 31,250 standard reactions (11969048001)
  sufficient for 6,250 standard reactions (11934546001)
packaging   pkg of 1,250 μL (11969048001 [100 mM])
  pkg of 4 × 1,250 μL (03732711001 [4x100 mM])
  pkg of 250 μL (11934546001 [100 mM])
mfr. no.   Roche
concentration   100 mM
shipped in   dry ice
storage temp.   −20°C
SMILES string   [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
InChI   1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key   KAELIXTVSANDSE-UHFFFAOYSA-N

Description

General description

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Application

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dTTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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