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EXPPCRKB Roche

KAPA Express Extract

with PCR ReadyMix

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Properties

Related Categories Genomic DNA Amplification, Molecular Biology, PCR/Amplification, from Mammalian Tissue
usage   sufficient for 100 reactions
  sufficient for 500 reactions
shelf life   ≤12 mo.
packaging   pkg of 1000 U (KK7152)
  pkg of 200 U (KK7151)
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

• KAPA Express Extract Enzyme (1 U/μL)
• 10X KAPA Express Extract Buffer
• 2X KAPA2G Robust HotStart ReadyMix

Preparation Note

Handling:
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.

Quality

All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.

Application

KAPA Express Extract with PCR ReadyMix is ideally suited for the extraction of PCR-ready DNA from, but not limited to, the following sample types:
• Human tissue (FFPE samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples)
• Animal samples (ear or tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
• Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples)
• Insects (crushed)
• Bird feathers (calamus fragments).

Features and Benefits

Key Features:
• Rapid extraction protocol: PCR-ready DNA in 15 minutes
• Versatility: single kit optimized for a variety of sample types
• Single-tube reaction minimizes risk of contamination

Rapid DNA extraction from a variety of sample types:
• From sample to PCR in less than 15 minutes
• Successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers

Increased PCR success from FFPE samples or tissues:
• Rapid DNA extraction and consistent downstream amplification of challenging sample types

Routine DNA extraction from blood sample types:
• Efficient extraction from “Guthrie”cards, FTAElute cards, and FTA cards

Quick Notes:
• Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
• Significantly reduced turnaround times and risk of sample loss and contamination.
• Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
• One extract typically yields a sufficient amount of template for 50–500 PCRs.
• DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
• KAPA2G Robust HotStart ReadyMix is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
• No quantification of DNA required prior to PCR.

General description

KAPA Express Extract with PCR ReadyMix is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.

The combination of KAPA Express Extract and KAPA2G Robust HotStart ReadyMix provides a high performance solution for rapid DNA extraction and consistent downstream amplification.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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