• USA Home
  • EXPPCRKB - KAPA Express Extract


KAPA Express Extract

with PCR ReadyMix



Related Categories Genomic DNA Amplification, Molecular Biology, PCR/Amplification, from Mammalian Tissue
usage   sufficient for 100 reactions
  sufficient for 500 reactions
shelf life   ≤12 mo.
packaging   pkg of 1000 U (KK7152)
  pkg of 200 U (KK7151)
shipped in   dry ice
storage temp.   −20°C



• KAPA Express Extract Enzyme (1 U/μL)
• 10X KAPA Express Extract Buffer
• 2X KAPA2G Robust HotStart ReadyMix

Preparation Note

KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.


All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.


KAPA Express Extract with PCR ReadyMix is ideally suited for the extraction of PCR-ready DNA from, but not limited to, the following sample types:
• Human tissue (FFPE samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples)
• Animal samples (ear or tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
• Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples)
• Insects (crushed)
• Bird feathers (calamus fragments).

Features and Benefits

Key Features:
• Rapid extraction protocol: PCR-ready DNA in 15 minutes
• Versatility: single kit optimized for a variety of sample types
• Single-tube reaction minimizes risk of contamination

Rapid DNA extraction from a variety of sample types:
• From sample to PCR in less than 15 minutes
• Successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers

Increased PCR success from FFPE samples or tissues:
• Rapid DNA extraction and consistent downstream amplification of challenging sample types

Routine DNA extraction from blood sample types:
• Efficient extraction from “Guthrie”cards, FTAElute cards, and FTA cards

Quick Notes:
• Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
• Significantly reduced turnaround times and risk of sample loss and contamination.
• Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
• One extract typically yields a sufficient amount of template for 50–500 PCRs.
• DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
• KAPA2G Robust HotStart ReadyMix is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
• No quantification of DNA required prior to PCR.

General description

KAPA Express Extract with PCR ReadyMix is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.

The combination of KAPA Express Extract and KAPA2G Robust HotStart ReadyMix provides a high performance solution for rapid DNA extraction and consistent downstream amplification.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


Directed Evolution

Proteins, the functional workhorses of living organisms, are composed of chains of interlinked amino acids that together form peptides and ultimately dictate the three-dimensional structure and funct...

KAPA Express Extract Kit FAQs

For which applications should I use the KAPA Express Extract Kit? The KAPA Express Extract Kit is ideally suited for the extraction of DNA from crude samples for most PCR applications, including qPCR...
Keywords: Amplification, Centrifugation, Degradations, Forensic, Nucleic acid annealing, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation

Kapa Biosystems Taq DNA Polymerase and PCR Kits

DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. Kapa Biosystems offers a robust portfolio of DNA polymerase reage...
Keywords: Amplification, Centrifugation, Cloning, DNA purification, Gas chromatography, Genetic, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Purification, Reductions, Sample preparations, Sequencing, Size-exclusion chromatography, Solvents

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?