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KK1024 Roche

KAPA Taq ReadyMix with dye

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Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
usage   50 μL sufficient for 250 reactions
shelf life   ≤18 mo.
packaging   pkg of 6.25 mL
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

• KAPA Taq Standard or HotStart DNA Polymerase 5 U/μL
• 10X KAPA Taq Buffer A
• 10X KAPA Taq Buffer B
• 10X KAPA Buffer with loading dye (optional)
• 5X KAPA Taq HotStart Buffer (HotStart kits only)
• MgCl2 (25 mM)
• dNTP Mix (10 mM each; optional)

Preparation Note

Handling:
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Quality

Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq ReadyMixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Application

KAPA Taq ReadyMix with dye has been used in:
• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Nested PCR (nPCR)

Features and Benefits

Key Features:
High performance:

• Improved sensitivity, specificity, and yields
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product

Quick Notes:
• KAPA Taq ReadyMix can replace any commercial Taq DNA polymerase in an existing protocol. The annealing temperature may need to be optimized to account for differences in formulation.
• The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
• The 2X KAPA Taq ReadyMix with dye includes two inert tracking dyes, which allow loading of PCR products directly onto agarose gels for analysis.
• KAPA Taq ReadyMixes contain 1.5 mM MgCl2 and 0.2 mM of each dNTP (at 1X).

General description

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5→′3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Taq is supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

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Peer-Reviewed Papers
15

References

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