EMAIL THIS PAGE TO A FRIEND
PFABIKB Roche

KAPA PROBE FAST

Purchase

Properties

shelf life   ≤12 mo.
packaging   kit of 1 mL (100 x 20 μL rxn; KK4705)
  kit of 10 mL (1000 x 20 μL rxn; KK4707)
  kit of 5 mL (500 x 20 μL rxn; KK4706)
  kit of 50 mL (5000 x 20 mL rxn; KK4714)
  pkg of
shipped in   wet ice
storage temp.   −20°C

Description

Packaging

Kits include KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM®, which contains KAPA Taq HotStart DNA Polymerase, reaction buffer, dNTPs, and MgCl2 at a final concentration of 5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both at 50X) separately.

Preparation Note

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM® is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

Quality

KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM® is subjected to stringent quality control tests, is free of contaminating exo and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Application

KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM® can be used for:
• Genotyping
• Gene expression analysis
• Multiplex qPCR
• SNP genotyping/allelic discrimination; and
• Microarray validation.
• RT-PCR/RT-qPCR

Features and Benefits

Accurately discriminate between alleles:
• Exceptional differentiation of heterozygous and homozygous alleles
• Discrete clusters in SNP genotyping assays

Achieve excellent reproducibility and efficiency:
• Fast, high performance five-colored multiplex PCR
• Similar abundance levels achieved with low copy number
• Broad dynamic range of up to 10 orders of magnitude

Increase flexibility:
• Compatible with both standard and fast cycling protocolsRetain accurate quantification

Quick Notes:
• These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
• Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
• For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
• For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
• Do not exceed 25 μL volumes.

General description

KAPA PROBE FAST qPCR Master Mix (2X) ABI Prism® Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).

Legal Information

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Prism is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Directed Evolution

Proteins, the functional workhorses of living organisms, are composed of chains of interlinked amino acids that together form peptides and ultimately dictate the three-dimensional structure and funct...

KAPA PROBE FAST qPCR Kits FAQs

What is the enzyme in KAPA PROBE FAST qPCR Master Mix (2X) Kits? This product contains KAPA Taq HotStart DNA Polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibod...
Keywords: Amplification, Degradations, Gene expression, Microarray Analysis, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reactions require reagents that are accurate and reliable. Kapa Biosystems qPCR and qRT-PCR reagents and kits are designed to provide consistent results for even t...
Keywords: Amplification, DNA purification, Digestions, Gas chromatography, Gene expression, Gene knockdown, Genetic, Melting, Methylations, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Size-exclusion chromatography

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?