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PFORCEKB Roche

KAPA PROBE FORCE

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Properties

usage   20 μL sufficient for 100 reactions
  20 μL sufficient for 500 reactions
shelf life   ≤12 mo.
packaging   kit of 1 mL (100 x 20 μL rxn; KK4300)
  kit of 5 mL (500 x 20 μL rxn; KK4301)
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

Universal (ROX pre-mixed).

Preparation Note

ROX reference dye is sensitive to light exposure. Always ensure that the product has been fully thawed and mixed before use. Avoid repeated freezing and thawing.

Quality

KAPA PROBE FORCE qPCR Master Mix Universal is subjected to stringent functional quality control, free of detectable contaminating exo- and endonuclease activities, and meets strict requirements with respect to DNA contamination.

Application

KAPA PROBE FORCE has been used for:
• GMO testing
• Mouse transgenics
• SNP genotyping
• Food/water pathogen detection
• Infectious disease research
• Cancer research
• DNA quantification.

Features and Benefits

Streamline sample-to-Cq workflows in <1 hour:
• Eliminate the time and cost of sample purification by amplifying directly from crude samples
• Analyze a wide range of sample types including whole blood, cells, mouse tails, FFPE, leaf, stem, seed, and soil

Generate accurate and reproducible results:
• Kits include a third-generation DNA polymerase, evolved for robust target amplification and detection
• Enzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generation

Break through high levels of qPCR inhibitors:
• Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samples
• Convert purified DNA assays to crude workflows without observable Cq delays

Multiplex crude samples efficiently:
• Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extracts
• Maximize data collection from precious samples, increase throughput, and reduce costs

Quick Notes:
• This kit contains the KAPA3G HotStart DNA Polymerase enzyme, enabling probe-based qPCR for both routine and challenging sample types.
• Initial denaturation of 3 min at 98°C is recommended to ensure complete denaturation of complex target DNA. A 5-min denaturation time may be required for some crude samples.
• For two-step cycling, use a 20-sec combined annealing/extension/data acquisition at 60°C as a first approach.
• A 10-sec annealing/extension/data acquisition time may be used with most assays, but this must be determined empirically.
• For crude samples, the amount of sample in the reaction may be reduced to improve performance, but this must be determined empirically.

General description

KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.
• Direct qPCR from crude blood, tissue, and plant extracts
• Sample-to-Cq workflows in <1 hour
• High efficiency for accurate, reproducible, and sensitive results
• Superior tolerance to carry-over inhibitors
• Multiplex compatibility with crude extracts

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Directed Evolution

Proteins, the functional workhorses of living organisms, are composed of chains of interlinked amino acids that together form peptides and ultimately dictate the three-dimensional structure and funct...

KAPA PROBE FORCE qPCR Kits

What is the enzyme in KAPA PROBE FORCE? This product contains a third-generation DNA polymerase, evolved to overcome the effect of PCR inhibitors. This DNA polymerase also enables very fast reaction ...
Keywords: Amplification, Buffers, Cancer, Environmental, Gas chromatography, Gene expression, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transgenics

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reactions require reagents that are accurate and reliable. Kapa Biosystems qPCR and qRT-PCR reagents and kits are designed to provide consistent results for even t...
Keywords: Amplification, DNA purification, Digestions, Gas chromatography, Gene expression, Gene knockdown, Genetic, Melting, Methylations, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Size-exclusion chromatography

Peer-Reviewed Papers
15

References

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