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KAPA Taq PCR Kit

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Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
shelf life   ≤18 mo.
packaging   pkg of 250 U (KK1014)
  pkg of 2500 U (BK1000)
  pkg of 500 U (KK1015)
  pkg of 5000 U (BK1002)
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

KAPA Taq PCR Kit includes the following components:
• KAPA Taq Standard or HotStart DNA Polymerase 5 U/μL
• 10X KAPA Taq Buffer A
• 10X KAPA Taq Buffer B
• 10X KAPA Buffer with loading dye (optional)
• 5X KAPA Taq HotStart Buffer (HotStart kits only)
• MgCl2 (25 mM)
• dNTP Mix (10 mM each; optional)

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.

Quality

Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Application

Existing appl- KAPA Taq PCR Kit has been used for:
• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Features and Benefits

High performance:
• Improved sensitivity, specificity, and yields
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product

Quick Notes:
• KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
• The final MgCl2 concentration may need to be optimized to account for differences in buffer formulation.
• KAPA Taq Buffers contain MgCl2 at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
• The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

General description

KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Taq is supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template—simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


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References

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