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ATPAM-RO Roche

AptaTaq Fast PCR Master

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Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
usage   sufficient for 1,000 reactions (06879101001)
  sufficient for 100 reactions (06879080001)
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Contents
Cat. No.: 06 879 080 001
1 x 400 μl for up to 100 reactions of 20 μl final reaction volume
Cat. No.: 06 879 101 001
10 x 400 μl for up to 1000 reactions of 20 μl final reaction volume

AptaTaq Fast PCR Polymerase is a reversible, temperature-dependent hot start DNA amplification system with immediate activation. Similar to antibody-based methods, the enzyme is activated by heating. Dropping the temperature below +55°C acts like a switch, shutting off polymerase activity. At temperatures above +60°C, AptaTaq Polymerase is activated, ideal for specific priming and fast PCR.

Kit for fast end-point PCR

AptaTaq Fast PCR Master, 5x concentrated, contains all reagents, except primers and nucleic acid template, needed for fast end-point polymerase chain reaction assays. Enzyme and master mix composition are optimized for fast activation and short PCR reaction times. AptaTaq Fast PCR Master contains AptaTaq Fast DNA Polymerase for hot-start PCR with immediate activation to improve specificity by minimizing the formation of non-specific amplification products.

The AptaTaq Fast PCR Master contains a thermostable, recombinant Taq polymerase with aptamer mediated, reversible hot start. It is immediate activated by heating, ideal for specific priming and fast PCR. Comes with optimized buffer system. Allows reaction setup at room temperature. A one-for-all protocol delivers superb results in less than 30 min for a broad range of templates incl. GC-rich templates.

Application

The AptaTaq Fast PCR Master contains a thermostable, recombinant Taq polymerase with aptamer mediated, reversible hot start & immediate activation. Similar to antibody-based methods, the enzyme is activated by heating, ideal for specific priming and fast PCR.
• Fast Hot Start PCR optimized for PCR products up to 500 bp
• Fast Hot Start RT-PCR optimized for PCR products up to 500 bp

Features and Benefits

Save time
• 4 minutes processing time only.
• Immediate enzyme activation.
Amplify with Confidence
• One-for-all protocol.
• Room temperature setup.
• Prevent carry-over contamination.
AptaTaq Fast Polymerase & Master redefine fast PCR:
AptaTaq Fast products are ready-to-use reagents optimized for fast PCR on all thermal cyclers. Inbuilt aptamer mediated hot start assures high specificity.

Unit Definition

One unit AptaTaq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of deoxyribonucleosidetriphosphates into acid precipitable DNA within 60 min at +65 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>80: NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,670,637, 5,693,502, and corresponding patent claims outside the US.  No right under any other patent claim is conveyed expressly, by implication, or by estoppel.

AptaTaq is a trademark of Roche

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

Protocols

AptaTaq Fast PCR Master Protocol & Troubleshooting

Water, PCR Grade (vial 2); 12 µl Forward primer, 10 µM; 1 µl; 0.5 µM Reverse primer, 10 µM; 1 µl; 0.5 µM AptaTaq Fast PCR Master, 5x concentrated (vial 1); 4µl; 1x concentrated
Keywords: Amplification, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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