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CDP-RO Roche

CDP-Star®

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

Synonym: CDP-Star® Chemiluminescent Substrate, Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate, ready-to-use, cdp-star

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Properties

Related Categories Chemiluminescent, Molecular Biology, Nucleic Acid Detection Substrates and Reagents, Nucleic Acid Detection and Hybridization
InChI Key   UKWLRLAKGMZXJC-VCCHNUGISA-L
assay   >98%
form   solution
packaging   bottle of 1 mL (11685627001[25 mM])
  bottle of 2 × 1 mL (11759051001[25 mM])
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Contents
25 mM solution (12.38 mg/ml), 100x concentration

Application

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
• Southern blot
• Northern blot
• Dot blot
• Colony and plaque hybridization
• Gel shift assays

Features and Benefits

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
• Save time with a fast, ultra-sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.
• Reuse the substrate by filtering and storing in sodium azide.

Quality

Purity: Purified by HPLC.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Other Notes

• First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
• No preincubation step required.
• Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
• Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
• Exposure times are shorter than with CSPD.
• Do not use nitrocellulose membranes.
• Repeat exposures can be made up to two days after the addition of substrate.

For life science research only. Not for use in diagnostic procedures.

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

12041677001 CDP-Star®, ready-to-use, Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

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