CSPD ready-to-use

Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [,7]decan}-4-yl)phenyl phosphate

Synonym: ready-to-use, cspd



Related Categories Chemiluminescent, DIG System Antibodies, Molecular Biology, Nucleic Acid Detection Substrates and Reagents, Nucleic Acid Detection and Hybridization,
Quality Level   100
assay   >98% (HPLC)
form   solution
mol wt   Mr 461
packaging   pkg of 1 mL (11655884001[25mM])
  pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C


General description

CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.


CSPD ready-to-use has been used for:
• the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
• colony and plaque hybridization
• gel shift assay
• sequencing
It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.

Features and Benefits

• Save time with a fast, highly sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.
• Reuse the substrate by filtering and storing in sodium azide.

Physical form

25mM solution (11.6mg/ml), 100x concentrated, colorless solution.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


CSPD ready-to-use Troubleshooting

Inefficient probe labeling Check labeling efficiency of your DIG DNA or RNA labeling by comparison to the labeled control DNA or RNA.
Keywords: Nucleic acid hybridization, Precipitation

Peer-Reviewed Papers


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