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DNALIG-RO Roche

T4 DNA Ligase

recombinant form of the enzyme from T4 phage

Synonym: dna ligase, t4

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Properties

Quality Level   100
form   solution
specific activity activity   ~3000 units/mg protein
packaging   pkg of 100 U (10481220001 [1 U/μl])
  pkg of 500 U (10716359001 [1 U/μl])
  pkg of 500 U (10799009001 [5 U/μl])
mfr. no.   Roche
parameter   4-22 °C optimum reaction temp.
optimum pH   7.2-7.8
shipped in   dry ice
storage temp.   −20°C

Description

General description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.

Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.

Specificity

Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).

Application

Ligation of sticky- and blunt-ended DNA fragments.

Quality

Quality

Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.

Unit Definition

One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C).

Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml

Preparation Note

Activator: Mg2+ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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