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DNTP-RO Roche

Deoxynucleoside Triphosphate Set

PCR Grade, sodium salt

Synonym: datp, dctp, dgtp, dttp, set of datp, dctp, dgtp, and dttp, pcr grade

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Properties

Related Categories Deoxynucleotide Sets, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
assay   <0.9% (dNDP)
  99% (dNTP)
usage   sufficient for 37,500 reactions (03622614001)
  sufficient for 6,250 reactions (11969064001)
packaging   pkg of 4 × 1,250 μL (03622614001 [4x100 mM])
  pkg of 4 × 250 μL (11969064001 [4x100 mM])
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 set containing 4 components
• dATP, PCR Grade, sodium salt, 100 mM
• dCTP, PCR Grade, sodium salt, 100 mM
• dGTP, PCR Grade, sodium salt, 100 mM
• dTTP, PCR Grade, sodium salt, 100 mM

Preparation Note

Working solution: For best results, prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Quality

Function tested using RT-PCR: Tested for absence of contaminating deoxyribonucleases, ribonucleases, and nicking activities according to the current Quality Control procedures.

Application

The Deoxynucleoside Triphosphate Set, PCR Grade, is suitable for many applications where high-quality reagents are required. Such applications include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Features and Benefits

PCR Grade Nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.
• PCR Grade Nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
• They are provided as clear, colorless solutions in water at a concentration of 100 mM (pH 8.3).
• Due to the manufacturing process, PCR Grade Nucleotides are free of dNTPs with modified bases, as well as tetra- and pyrophosphate contamination. Tetraphosphates can disintegrate and form pyrophosphate, which is a known inhibitor of amplification reactions. Therefore, the purity of nucleotides is critical to the success of your amplification reactions.
• PCR Grade Nucleotides are stable for 30 months after the production date when stored at -15 to -25°C.

General description

This product contains 4 separate vials of dATP, PCR Grade, dCTP, PCR Grade, dGTP, PCR Grade, and dTTP, PCR Grade, each with a concentration of 100 mM and as a clear, colorless solution of the sodium salt (pH 8.3).

Kit component only

Description

Product #

Add to Cart

dATP, PCR Grade, sodium salt 100 mM    
dCTP, PCR Grade, sodium salt 100 mM    
dGTP, PCR Grade, sodium salt 100 mM    
dTTP, PCR Grade, sodium salt 100 mM    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles

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Peer-Reviewed Papers
15

References

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