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DTT-RO Roche

DTT

1,4-Dithiothreitol

Synonym: 1,4-dithiothreitol, threo-1,4-Dimercapto-2,3-butanediol, DL-Dithiothreitol, Cleland’s reagent, DTT, cleland′s reagent, dithiothreitol, 1,4-

  • CAS Number 3483-12-3

  • Linear Formula HSCH2CH(OH)CH(OH)CH2SH

  • Molecular Weight 154.25

  •  Beilstein/REAXYS Number 1719757

  •  MDL number MFCD00004877

  •  PubChem Substance ID 329798930

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Properties

Related Categories Chemical Synthesis, Other Synthetic Reagents for Reduction, Reduction, Synthetic Reagents
Quality Level   100
InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
assay   ≥97% (Ellman′s reagent)
form   crystalline powder
mol wt   Mr 154.3
packaging   pkg of 10 g (10708984001)
  pkg of 2 g (10197777001)
  pkg of 25 g (11583786001)
mfr. no.   Roche
mp   41-44 °C (lit.)
shipped in   wet ice
storage temp.   2-8°C

Description

General description

1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality.

Application

1,4-dithiothreitol is used in:
• Isolation, purification and characterization of proteins and enzymes
• Measurement of enzyme activities (reactivation of enzymes)
• Determination of disulfide groups in proteins and enzymes
• DNA extraction prior to amplification

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Specifications

Oxidized form: <2.5% (absorbance at 283nm)

Sequence

Enantiomers
Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT.

Preparation Note

Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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