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Expand High FidelityPLUS PCR System

Synonym: high fidelity pcr plus system, expand

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Immunoassays, Fluorescent Probes, Labels, Particles and Stains
Quality Level   100
usage   sufficient for ≤2,500 reactions (03300234001)
  sufficient for ≤500 reactions (03300226001)
packaging   pkg of 2,500 U (03300234001 [10x250 U])
  pkg of 500 U (03300226001 [2x250 U])
mfr. no.   Roche
parameter   72 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Application

The Expand High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:
• PCR
• RT-PCR
• Primer extension
• Carryover prevention

Features and Benefits

• Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
• Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
• Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Packaging

1 kit containing 4 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μL

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.

Expand is a trademark of Roche

Kit component only

Description

Product #

Add to Cart

Expand High FidelityPLUS Enzyme Blend 5 U/μl    
Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated    
Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated    
MgCl2 Stock Solution 25 mM    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Expand™ High FidelityPLUS PCR System Troubleshooting

ELEVATED ERROR RATES The typical error rate (fidelity) of Taq DNA Polymerase, determined by the lacI-based PCR fidelity assay, ranges from 1 × 10-5 to 2.5 × 10-5. Roche has determined an error rate o...
Keywords: Amplification, Polymerase chain reaction, Solvents, Titrations

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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