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Endoproteinase Glu-C Sequencing Grade

from Staphylococcus aureus V8



Related Categories Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteolytic Enzymes, Proteolytic Enzymes and Substrates
form   lyophilized (salt-free)
specific activity activity   ≥15 units/mg protein
mol wt   Mr 27 kDa
packaging   pkg of 3 × 50 μg (11047817001)
  pkg of 50 μg (11420399001)
mfr. no.   Roche
optimum pH   4.0 - 7.8
shipped in   wet ice
storage temp.   2-8°C


Features and Benefits

Free of impurities that may interfere with separation of peptides in RP HPLC.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): -15 to -25 °C
At least 2 months if stored frozen in portions.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.


Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.


Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.


Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.

General description

Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.

Price and Availability

Biomedical Applications
Safety & Documentation

Safety Information

Signal word 
Hazard statements 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers


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