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FIBRP-RO Roche

Fibronectin (pure)

Synonym: Fibronectin

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Properties

Related Categories Attachment Factors, Cell Culture, Reagents and Supplements More...
biological source   human plasma
sterility   non-sterile; 0.2 μm filtered
assay   >95% (SDS-PAGE)
  >95% (SDS-PAGE)
form   lyophilized (clear, colorless solution after reconstitution)
packaging   pkg of 1 mg (11051407001)
  pkg of 5 mg (11080938001)
mfr. no.   Roche
suitability   suitable for cell culture
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... FN1(2335)

Description

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Specificity

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Physical form

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Sequence

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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