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FSSGMMRO Roche

FastStart SYBR Green Master

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Properties

Quality Level   100
mfr. no.   Roche
packaging   pkg of 500 x 20 μL reactions (04673484001)
  pkg of 5000 x 20 μL reactions (04673492001)

Description

Features and Benefits

• Improve PCR sensitivity and specificity:
Rely on this mix′s FastStart Taq DNA Polymerase to minimize the formation of nonspecific amplification products through hot start PCR.
• Avoid over-estimation of qPCR results:
Eliminate nonspecific amplification products and primer-dimers that would increase the amount of bound quantified SYBR Green I.
• Amplify and detect a broad range of DNA or cDNA targets:
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich. Works with any real-time PCR instrument other than the LightCycler® Instruments.
• Use any real-time PCR instrument other than the LightCycler® Instruments:
Choose from two formulations - one that contains the ROX reference dye and one without ROX.
• Save time and effort in qPCR preparation:
Rely on this easy-to-use 2x master mix to eliminate the need to mix components, titrate MgCl2, or perform other time-consuming optimization steps.
• Prevent false positives resulting from carryover contamination:
The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

Components

FastStart SYBR Green Master, 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and SYBR Green I.

Quality

Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (about 440 bp).

Other Notes

Cancer Research - Gene Expression Analysis You Can Count On Article (PDF, 622 KB)

Gene Expression Analysis You Can Count On Article (PDF, 597.72 KB)

qPCR targets
In principle, the FastStart SYBR Green Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, for each target, you would need:

• to adapt your detection protocol to the reaction conditions of your real-time PCR instrument, and
• design specific PCR primers for the target.

See the Operator′s Manual of your real-time PCR instrument for general recommendations.
Two forms available
The master mix is available in two forms – one that contains the ROX reference dye and one without ROX.
Preventing carryover contamination
This master contains dUTP, which will be incorporated into PCR products to help prevent false positives resulting from carryover contamination. In subsequent PCRs, you can add Uracil-DNA Glycosylase to degrade any uracil-containing carryover contaminants (amplification products from previous PCRs).
qRT-PCR
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit for two-step qRT-PCR. This kit gives excellent results and works efficiently with all real-time PCR instruments.

For life science research only. Not for use in diagnostic procedures.

Legal Information

FastStart is a trademark of Roche

LightCycler is a registered trademark of Roche

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Hot Start PCR

Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows fo...
Keywords: Amplification, Buffers, Gas chromatography, Polymerase chain reaction, Polymerase chain reaction - quantitative

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PCR Master Mix

A PCR master mix, sometimes known as super mix or ready mix, is a batch mixture of PCR reagents at optimal concentrations that can be prepared and divided among many PCR tubes or 96-well PCR plates. ...
Keywords: Buffers, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription

Peer-Reviewed Papers
15

References

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