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FastStart Taq DNA Polymerase, 5 U/μl

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Related Categories Biochemicals and Reagents, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Immunoassays, Fluorescent Probes, Labels, Particles and Stains
usage   sufficient for ≤1,250 reactions (12032945001)
  sufficient for ≤2,500 reactions (12032953001)
  sufficient for ≤250 reactions (12032929001)
  sufficient for ≤50 reactions (12032902001)
  sufficient for ≤500 reactions (12032937001)
packaging   pkg of 1,000 U (12032937001 [4x250 U])
  pkg of 2,500 U (12032945001 [10x250 U])
  pkg of 5,000 U (12032953001 [20x250 U])
  pkg of 100 U (12032902001)
  pkg of 500 U (12032929001 [2x250 U])
mfr. no.   Roche
parameter   72 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C

Description

General description

FastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH Solution to enable the enzyme to handle a wide range of templates are also supplied.

Application

FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
It can be applied for:
• PCR
• Multiplex PCR
• Difficult templates e.g., secondary structures or GC-rich sequences
• Automated PCR e.g., handling at room temperatures
• Hot Start PCR up to 3kb
• Hot Start RT-PCR up to 3kb

Use FastStart Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2× concentrated ready-to-use FastStart PCR Master.

Features and Benefits

FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations.
• Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier.
• Use robotic setup.
Use this enyzme mix stable for 24hours at +15 to +25°C.
• Prevent PCR carryover contamination.
Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes.

Packaging

1 kit containing 5 components

Quality

Each lot is function-tested using human genomic DNA and primers specific for the 365 bp fragment of human tPA gene, and, a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exo- and endonucleases and nicking activity.

Unit Definition

1 μg M13mp9ss DNA, 0.3 μg M13 sequencing primer and 0.1 μCi [α-32P] dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Unit Assay: 1 μg M13mp9ss DNA, 0.3 μg M13 sequencing primer and 0.1 μCi [α-32P] dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

Preparation Note

Storage conditions (working solution): Fast Start Taq mastermix is stable for 4 weeks at -25 °C to -15 °C without primers
Please note: This parameter is not part of specification

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only.  Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

FastStart is a trademark of Roche

Kit component only

Description

Product #

Add to Cart

FastStart Taq DNA Polymerase, in storage and dilution buffer 5 U/μl    
PCR Reaction Buffer, with 20 mM MgCl2 10x concentrated    
PCR Reaction Buffer, without MgCl2 10x concentrated    
MgCl2 Stock Solution 25 mM    
GC-RICH Solution 5x concentrated    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Hot Start PCR

Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows fo...
Keywords: Amplification, Buffers, Gas chromatography, Polymerase chain reaction, Polymerase chain reaction - quantitative

Protocols

FastStart™ Taq DNA Polymerase, 5 U/μl Protocol & Troubleshooting

PCR OPTIMIZATION The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome. Template purity and quality are also critical to PCR success. Sequence and p...
Keywords: Amplification, Polymerase chain reaction

Reagents for PCR - Select the Best Roche Product for Your PCR Application

Cutting-edge science shouldn’t have to compromise for the sake of economy. Since the introduction of PCR technology, Roche has provided the gold standard in PCR reagents. Our advanced isolation, puri...
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

Peer-Reviewed Papers
15

References

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