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G418-RO Roche

G-418 Solution

Synonym: O-2-Amino-2,7-dideoxy-Δ-glycero-α-Δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-Δ-streptamine, disulfate salt, Antibiotic G418, G 418 disulfate salt solution, neomycin

  • CAS Number 108321-42-2

  • Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4

  • Molecular Weight 692.71

  •  MDL number MFCD00058314

  •  PubChem Substance ID 329799925

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Properties

Related Categories Antibiotic Solutions, Antibiotics, Cell Culture, Reagents and Supplements
Quality Level   100
assay   ≥98% (TLC)
form   solution
mol wt   692.7 Da
packaging   pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g])
  pkg of 20 mL (04727878001 [equivalent to 1 g])
mfr. no.   Roche
concentration   50 mg/mL
application(s)   transfection: suitable
Mode of action   protein synthesis | inhibits
antibiotic activity spectrum   protozoa
shipped in   wet ice
storage temp.   2-8°C
SMILES string   OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI   1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key   UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

General description

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

Application

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neor) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Features and Benefits

• Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
• Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
• Function tested in a cell culture assay - ensures effectiveness.
• Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
• Cost effective, since the selected colonies are stable.
Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Quality

Purity: = 98%, as shown by TLC.
Function testing: in cell culture.

Preparation Note

Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Protocols

G-418 Solution Protocol

OPTIMAL WORKING CONCENTRATION OF G-418 SOLUTION In this Biochemica article, the dose-response curve of different concentrations of G-418 solution were tested for their ability to stop growth of the a...
Keywords: Antibiotics, Cancer, Scintillation, Transfection

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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