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HBFGF-RO Roche

Basic Fibroblast Growth Factor, human (hbFGF)

recombinant (E. coli)

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Properties

Related Categories Cell Culture, FGF-2 (FGF basic), Fibroblast Growth Factors (FGF), Growth Factors and Cytokines, Reagents and Supplements More...
biological source   human
sterility   non-sterile; 0.2 μm filtered
assay   >95% (SDS-PAGE)
form   lyophilized (salt-free)
potency   <1.0 ng/mL
mol wt   mol wt 18,000 kDa
packaging   pkg of 10 μg (11123149001)
  pkg of 25 μg (11120417001)
mfr. no.   Roche
storage condition   avoid repeated freeze/thaw cycles
impurities   <0.1 EU/μg
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

Specific activity/EC50:<1 ng/ml, at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 0.5 to 10 ng/ml
For use in serum-free cell culture.
Working solution: Reconstitute the lyophilizate in sterile PBS (final concentration 100 μg/ml). Dilute further with PBS or medium containing 1 mg/ml BSA or 1 to 10% serum
Storage conditions (working solution): -15 to -25 °C
Reconstituted solution can be stored in aliquots at -15 to -25 °C.
Stable in culture at least for 3 days.
Note: Avoid repeated freezing and thawing.

Quality

Endotoxin level: <0.1 EU/μg (LAL-test)
Note: 1 EU corresponds to 0.1 ng

Specificity

Human bFGF is effective on mouse, bovine, and human cells.

Unit Definition

EC50 definition: The concentration of hbFGF that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with Balb/c 3T3 (NR6) cells.

Application

Basic fibroblast growth factor has been used as one of the components of EBM-B (endothelial Basal Medium-2 media, heat-inactivated fetal bovine serum, Glutamine-penicillin-streptomycin sulfate and basic fibroblast growth factor) media.

General description

Bovine bFGF has 146 amino acids. bFGF shares 55% amino acid homology with a second endothelial cell mitogen, acidic FGF (aFGF) (also known as endothelial cell growth factor, ECGF). The amino acid sequence for human bFGF is very similar to the sequence of the bovine protein. The proteins differ only by two amino acids. Both the human and bovine proteins appear to be synthesized initially with an amino-terminal extension of nine residues. bFGF is a potent mitogen for a wide variety of mesoderm- and neuroectoderm- derived cells including fibroblasts, vascular and corneal endothelial cells, myoblasts, chondrocytes, osteoblasts, smooth muscle cells, and glial cells. All cell types that respond to FGFs bear specific FGF cell surface receptors. In addition, bFGF induces blood vessel growth and wound healing in vivo. Other in vivo effects of bFGF are mesodermal induction in early embryos and limb and lens regeneration.

Sequence

Chain Length 155 AA

The primary structure of recombinant human bFGF (one polypeptide chain, 155 amino acids) is the biologically active precursor of natural human bFGF (one polypeptide chain, 146 amino acids), with the 9 additional amino acids located at the amino-terminus.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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