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HIL2-RO Roche

Interleukin-2, human (hIL-2)

recombinant (E. coli)

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones,
Quality Level   100
biological source   human
recombinant   expressed in E. coli
assay   95% (SDS-PAGE)
form   solution
potency   >2 x 10^6 units/mg EC50
mol wt   (15,000 Da)
packaging   pkg of 10,000 U (10799068001 [5 μg, 50 ml])
  pkg of 10,000 U (11011456001 [5 μg, 1 ml])
  pkg of 50,000 U (11147528001 [25 μg, 5 ml])
mfr. no.   Roche
storage condition   avoid repeated freeze/thaw cycles
concentration   10-20U/ml
impurities   <0.1 EU/μg
UniProt accession no.   P60568
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... IL2(3558)

Description

General description

Recombinant Interleukin-2, human (hIL-2) is produced in E. coli and purified by standard chromatographic techniques. Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane.

Specificity

Species specificity: Recombinant IL-2, human is effective on mouse and human cells.

Application

Recombinant IL-2, human, allows:
• The cultivation of human and murine IL-2 dependent T-cell lines and natural killer cell lines.
• The proliferation of mitogen-activated T-lymphocytes and natural killer cells.
• The establishment of human and murine thymocyte, splenocyte, or peripheral blood lymphocyte (PBL) derived T-cell lines.
• The generation of human and murine lymphokine-activated killer (LAK) cells (1-11).
• In vitro re-stimulation of lung cells obtained from mice.

Biochem/physiol Actions

Interlekin-2 (IL-2) is a potent pro-inflammatory cytokine. It plays a protective role in autoimmune chronic inflammation, when present in low amounts. It is involved in maintaining immune homeostasis where it controls the cross-talk between T regulatory and T effector cells. Studies in acute myelogenous leukemia (AML) mice models show that antibody based delivery of IL-2 to targets present in tumor-linked vasculature results in antileukemic activity.

Quality

Purity: >95% pure as determined by SDS-PAGE
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(1 EU corresponds to 0.1 ng)

Sequence

Chain Length 134 AA

The primary structure of recombinant, IL-2, human is identical to that of natural, human IL-2 (one polypeptide chain, 133 amino acids), however, recombinant IL-2 has an extra methionine at the amino-terminus (one polypeptide chain, 134 amino acids) and is not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC50 definition: The amount of hIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).

Unit Conversion: 1 BM unit = 3.25 NBSB units (natural IL-2)1 BM unit = 3.25 NBSB units (natural IL-2)

Physical form

Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane

Preparation Note

Working concentration: 10 -20 U/ml
Established IL-2-dependent T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependent cell lines.

Working solution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1 to 10% serum.

Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Specific activity/EC 50 : >2 x 106 U/mg <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50 ) compared to the indicated standard is guaranteed (19, 20). Human, recombinant IL-2 has the same biological activity in vitro as compared to human, natural IL-2 (15, 1–4).

Recommended Method of Dilution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1–10% serum.
Interleukin-2 (IL-2, also known as T-Cell Growth Factor, TCGF) is a lymphokine which is produced by lectin- or antigen-activated T-cells and plays an important immunoregulatory role. This factor, or lymphokine, was first identified by its ability to promote the long-term in vitro proliferation of activated T cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine- activated killer (LAK) cells (1–11). Recombinant human IL-2 allows the cultivation of human and murine IL-2-dependent T-cell lines and natural killer cell lines, the proliferation of mitogenactivated T-lymphocytes and natural killer cells, the establishment of human and murine thymocyte-, splenocyte-, or peripheral blood lymphocyte (PBL)- derived T-cell lines, and the generation of human and murine lymphokine-activated killer (LAK) cells (1-11). Established IL-2-dependant T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependant cell lines.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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References

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