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KK4752 Roche

KAPA PROBE FAST One-Step

Universal

  •  NACRES NA.55

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Properties

Related Categories Molecular Biology, PCR/Amplification, RT-PCR and RT-qPCR More...
Quality Level   100
packaging   pkg of 500 RXNS
storage temp.   −20°C

Description

General description

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes.

Application

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:
• Gene expression analysis
• SNP/mutation analysis
• Gene knockdown validation
• RT-PCR

Features and Benefits

Quick Notes:
• The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
• Use only gene-specific primers for one-step qRT-PCR.
• Optimal cDNA synthesis is achieved at 42°C for 5 min.
• 3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
• Do not exceed 25 μL reaction volumes

Packaging

Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)

Quality

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

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Protocols

SARS-CoV-2: qPCR, RT-PCR Reagents and Assay Design

We are working hand in hand with public health authorities and customers to support the fight against novel coronavirus (SARS-CoV-2). In response to the community’s need for SARS-CoV-2-specific reage...
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PCR Selection Guide

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Peer-Reviewed Papers
15

References

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