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NAD100-RO Roche

NAD

Grade I, free acid

Synonym: β-DPN, β-NAD, β-Nicotinamide adenine dinucleotide hydrate, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide

  • CAS Number 53-84-9

  • Empirical Formula (Hill Notation) C21H27N7O14P2 · xH2O

  • Molecular Weight 663.43 (anhydrous basis)

  •  MDL number MFCD00036253

  •  PubChem Substance ID 329818699

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Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Oxidation-Reduction
InChI Key   BAWFJGJZGIEFAR-NNYOXOHSSA-N
assay   100% (dry weight)
form   lyophilized
mol wt   Mr 663.4
packaging   pkg of 1 g (10127965001)
  pkg of 5 g (10127973001)
mfr. no.   Roche
color   off-white
pH   ~3.0 (50 mg/mL in water)
absorption   Amax 260 nm
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

Application

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

Biochem/physiol Actions

Electron acceptor

Packaging

Packaged by solid weight.

Quality

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A250nm - A260nm : 0.83 ± 0.02, A280nm - A260nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Specifications

Absorbance coefficients: 17.6 x 103l x mol-1 x cm-1 at 260nm, 5.9 x 103l x mol-1 x cm-1 at 333nm (CN complex)

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

This is the common form of NAD.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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