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NAD98-RO Roche

NAD

Grade II, free acid

Synonym: β-DPN, β-NAD, β-Nicotinamide adenine dinucleotide hydrate, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide

  • CAS Number 53-84-9

  • Empirical Formula (Hill Notation) C21H27N7O14P2 · xH2O

  • Molecular Weight 663.43 (anhydrous basis)

  •  MDL number MFCD00036253

  •  PubChem Substance ID 329818700

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Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Oxidation-Reduction
InChI Key   BAWFJGJZGIEFAR-NNYOXOHSSA-N
assay   98% (dry weight)
form   lyophilized
mol wt   Mr 663.4
packaging   pkg of 1 g (10127981001)
  pkg of 10 g (10621650001)
  pkg of 5 g (10127990001)
mfr. no.   Roche
color   off-white
pH   ~3.0 (50 mg/mL in water)
absorption   λmax 260 nm
shipped in   wet ice
storage temp.   2-8°C

Description

Biochem/physiol Actions

Electron acceptor

Packaging

Packaged by solid weight.

Quality

Absence of contaminants: LDH inhibitors are not detectable.
Organic solvents: <0.5% (GC)

Preparation Note

Storage conditions (working solution): An aqueous solution in a concentration of 10 mg/ml (pH 2.5 to 3) maintains > 99% of its NAD content after 8 weeks of storage at 2 to 8 °C.
The stability of an aqueous solution in a concentration of 40 mg/ml is not expected to differ from that of a solution in a concentration of 10 mg/ml.

An aqueous solution in a concentration of 50 mg/ml (pH 2.0) maintains 97% of its NAD content after 2 days, 94% after 6 days and 91% after 10 days of storage at 2 to 8 °C and about 83% after 10 days of storage at 33 °C.

An aqueous solution in a concentration of 1.38 mg/ml shows a decrease of NAD content of 4% after 8 weeks of storage at 2 to 8 °C, stored at -15 to -25 °C the stability is expected to be > 6 months. A solution of NAD in triethanolamine buffer (pH 5.0) maintians 90% of its NAD content after
10 days of storage at 2 to 8 °C and 80% after 10 days of storage at 33 °C.

Analysis Note

Absorbance coefficients: 17.6 x 103 l x mol-1 x cm-1 at 260 nm, 5.9 x 103 l x mol-1 x cm-1 at 333 nm (CN complex)

ß-Nicotinamide adenine dinucleotide hydrate cofactor (NAD) has been used in an experimental protocol, done in order to mimic the citric acid cycle on an carbon electrode, in order to increase biofuel cell energy density.

Other Notes

For life science research only. Not for use in diagnostic procedures.

This is the common form of NAD.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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