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NADP-RO Roche

NADP

Disodium salt

Synonym: β-NADH phosphate disodium salt, β-NADP-Na2, β-Nicotinamide adenine dinucleotide phosphate disodium salt, Triphosphopyridine nucleotide disodium salt

  • CAS Number 24292-60-2

  • Empirical Formula (Hill Notation) C21H26N7Na2O17P3

  • Molecular Weight 787.37

  •  MDL number MFCD00065390

  •  PubChem Substance ID 329818702

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Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Oxidation-Reduction
Quality Level   100
assay   ≥97%
form   powder
mol wt   Mr 743.4
  Mr 787.4
packaging   pkg of 1 g (10128058001)
  pkg of 100 mg (10128031001)
  pkg of 5 g (10240354001)
  pkg of 500 mg (10128040001)
mfr. no.   Roche
pH   4.0 ± 1.0(10mg/mL)
λmax   260 nm
shipped in   wet ice
storage temp.   2-8°C
SMILES string   [Na+].[Na+].NC(=O)c1ccc[n+](c1)C2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](OP(O)([O-])=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
InChI   1S/C21H28N7O17P3.2Na/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39);;/q;2*+1/p-2/t10-,11-,13-,14-,15-,16-,20?,21-;;/m1../s1
InChI key   WSDDJLMGYRLUKR-FHQWUTKFSA-L

Description

General description

Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair. The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.

Application

The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen. It has also been used for the hexokinase assay.

Preparation Note

Storage conditions (working solution): 2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C

Analysis Note

Absorbance coefficients: 18.0 x 103 [l x mol-1 x cm-1] at 260 nm, 5.9 x 103 [l x mol-1 x cm-1] at 333 nm (CN complex)

Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A340 approximately 0.100.

Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A360 approximately 0.200-0.250.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Phosphorylation

Phosphorylation is the addition of a phosphoryl (PO3) group to a molecule. In biological systems, this reaction is vital for the cellular storage and transfer of free energy using energy carrier mole...
Keywords: Aerobic, Anaerobic, Cellular processes, Glycolysis, Oxidations, Phosphorylations, Reductions, Respiration

Peer-Reviewed Papers
15

References

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