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NADP-RO Roche

NADP

Disodium salt

Synonym: β-NADH phosphate disodium salt, β-NADP-Na2, β-Nicotinamide adenine dinucleotide phosphate disodium salt, Triphosphopyridine nucleotide disodium salt

  • CAS Number 24292-60-2

  • Empirical Formula (Hill Notation) C21H26N7Na2O17P3

  • Molecular Weight 787.37

  •  MDL number MFCD00065390

  •  PubChem Substance ID 329818702

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Properties

Related Categories Biochemicals and Reagents, Cofactors, Enzymes, Inhibitors, and Substrates, Oxidation-Reduction
InChI Key   WSDDJLMGYRLUKR-FHQWUTKFSA-L
assay   ≥97%
form   powder
mol wt   Mr 743.4
  Mr 787.4
packaging   pkg of 1 g (10128058001)
  pkg of 100 mg (10128031001)
  pkg of 5 g (10240354001)
  pkg of 500 mg (10128040001)
mfr. no.   Roche
pH   4.0 ± 1.0(10mg/mL)
absorption   Amax 260 nm
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair. The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.

Application

The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen. It has also been used for the hexokinase assay.

Preparation Note

Storage conditions (working solution): 2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C

Analysis Note

Absorbance coefficients: 18.0 x 103 [l x mol-1 x cm-1] at 260 nm, 5.9 x 103 [l x mol-1 x cm-1] at 333 nm (CN complex)

Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A340 approximately 0.100.

Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A360 approximately 0.200-0.250.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
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References

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