PCRH20-RO Roche

Water, PCR Grade



Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
Quality Level   100
form   liquid
packaging   pkg of 100 mL (03315843001 [4 x 25 ml])
  pkg of 25 mL (03315932001 [25 x 1 ml])
  pkg of 25 mL (03315959001 [1 x 25 ml])
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C


General description

Water, PCR Grade is specially purified, double-distilled, deionized, and autoclaved. The production process ensures that even according to Roche ′s strict Quality Control procedures the water does not contain detectable amounts of nucleic acid. It is routinely tested in PCR with conventional thermal cyclers and with LightCycler® Instruments.


This product is specially tested and qualified for use in all PCR or RT-PCR. It can be used in conjunction with all end-point or real-time PCR assays or whenever highest quality water is needed. All of the LightCycler® kits and some of the amplification products — available from Roche Applied Science — contain this Water, PCR Grade.


Function test: Each lot of Water, PCR Grade is tested in PCR (amplification of a 0.5 kb target from 0.01 ng λDNA). When the PCR product is analyzed by gel electrophoresis, a clear, defined band must be seen on the gel.
Absence of nucleases: The water is tested with various nucleic acids that could serve as nuclease substrates. No endonucleases, ribonucleases or nicking activity are detected according to the current Quality procedures.

Preparation Note

Storage conditions (working solution): Store a working solution for a short period of time (e.g. 1 week) at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

LightCycler is a registered trademark of Roche

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

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