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RNASEA-RO Roche

RNase A

from bovine pancreas

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Molecular Biology Tested, Protein and Nucleic Acid Isolation More...
form   dry powder
activity   50 U/mg ((+25°C, RNA as substrate))
mol wt   Mr = 13,700
packaging   pkg of 100 mg (10109169001)
  pkg of 25 mg (10109142001)
mfr. no.   Roche
parameter   65 °C optimum reaction temp.
optimum pH   7.0-7.5
shipped in   wet ice
storage temp.   2-8°C

Description

Application

• For analytical purposes
• Isolation of DNA (for this purpose, RNase A should be boiled)
• For cell cycle analysis by flow cytometry and propidium iodide (PI) staining

Other Notes

For life science research only. Not for use in diagnostic procedures.

Reconstitution

RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Biochem/physiol Actions

RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding’ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.

General description

Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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