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X-tremeGENE siRNA Transfection Reagent

Polymer reagent for delivering siRNA to common cell lines

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Properties

Related Categories Application: For delivery of siRNA to commonly used cells, Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology,
Quality Level   100
grade   for molecular biology
usage   sufficient for 2,000 transfections (04476115001)
  sufficient for 400 transfections (04476093001)
packaging   pkg of 1 mL (04476093001 [1 mg/ml])
  pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])
mfr. no.   Roche
concentration   1 mg/mL
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Short interfering RNA (siRNA) can be directly introduced into cells by transfection. A lipid-based transfection reagent, such as X-tremeGENE siRNA Transfection reagent, can provide a convenient, reliable, and efficient vehicle for delivering siRNAs into animal cells, enabling the study of cellular and functional consequences of gene knockdown.
This innovative reagent forms a complex with siRNA, as well as with mixtures of siRNA and plasmid DNA (cotransfection), and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps: mix and incubate diluted transfection reagent with diluted siRNA, then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity, it can be used without media changes. The product is animal-component free.

Contents

Solution filtered through 0.2 μm pore size membrane, supplied in polypropylene tubes.

Application

X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines, such as HT29, a human adenocarcinoma cell line.

Features and Benefits

• Knock down gene expression over 90% in many different cell types.
• Maximize experimental flexibility with a single reagent for siRNA- and cotransfection-based gene-knockdown experiments.
• Produce meaningful results using a reagent that exhibits low cytotoxicity, ensuring that the cellular effects you observe are due to the transfected siRNA rather than the transfection procedure.
• Work with or without serum, avoiding medium changes (e.g., to serum-free medium) before or after transfection.;

X-tremeGENE siRNA Transfection Reagent is a proprietary blend of lipids and other components, free of animal products.

Quality

Activity assay: X-tremeGENE siRNA Transfection Reagent (1 - 2.5 μl) is combined with siRNA (0.1 - 0.35 μg) that is specific for the HPRT housekeeping gene. The mixture is used to transfect HEK-293 cells (in a monolayer, 30 - 50% confluent) in the presence of 10% fetal bovine serum (FBS). Following transfection, the decrease of HPRT mRNA cells is determined with the LightCycler® Real-Time PCR System. A knockdown efficiency of 70 - 95% is typically observed when mRNA is measured.

Cytotoxicity analysis: HEK-293 cells are exposed to siRNA/X-tremeGENE siRNA Reagent complexes for 72 hours in the presence of serum, without a media change. Cytotoxicity is then tested by analyzing the cells with the WST-1 Cell Proliferation Reagent (Roche).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
Keywords: Antibiotics, Diagnostic, Gene expression, Genetics, Transfection, Western blot

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques serve as an analytical too...
Keywords: Apoptosis, Biological techniques, Drug discovery, Gene expression, Genetic, Precipitation, Transduction, Transfection, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Roche Transfection Database

X-tremeGENE™ Transfection Reagents from Roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells. Use the table below to find the right produ...
Keywords: Apoptosis, Cancer, Clinical, Cloning, DNA replication, Gene expression, Immunoprecipitation, In Situ hybridization, Individual protein Immunoprecipitation, Ion-exchange chromatography, Methylations, Microscopy, Nucleic acid hybridization, Polymerase chain reaction, Recombination, Transcription, Transfection, Western blot

Transfection Best Practices

Successful transfection of nucleic acids and proteins depends on numerous factors that range from reagent and cell type selection to protocol optimization. Ensure optimal quality in your experiment b...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Infrared spectroscopy, Ion-exchange chromatography, Transfection

X-tremeGENE™ Transfection Reagents General Recommendations

The ability to successfully transfect a cell varies significantly depending on the cell type, protocol, and the composition of the transfection reagent. X-tremeGENE™ transfection reagents are designe...
Keywords: Cancer, Cell culture, Cellular processes, Diagnostic, Gene expression, Gene knockdown, Microscopy, Transfection

X-tremeGENE™ siRNA Transfection Reagent: A Powerful Tool for Antisense Inhibition of miRNA in Human Cells with miRCURY Knockdown Probes

MicroRNAs (miRNAs) are an endogenous class of RNA molecules comprising 19-25 nucleotides that regulate the suitability or transflational efficiency of mRNAs. The mature miRNAs are processed from long...
Keywords: Apoptosis, Cancer, Cell proliferation, Diagnostic, Gene expression, In Situ hybridization, Nucleic acid hybridization, Transfection

Protocols

Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ transfection reagents provide reliable transfection of diverse nucleic acids and CRISPR/Cas9 components, a critical step for your molecular and cellular research. Explore our list of the...
Keywords: Diagnostic, Gene expression, Transfection

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

siRNA IN PRIMARY LUNG FIBROBLASTS TRANSFECTION Roche has no data for this application, but there is a report from a customer, briefly described below. Wild rat lung fibroblasts were successfully cotr...
Keywords: Gene expression, Growth factors, Microarray Analysis, Transfection, Western blot

Related Content

How Transfection Works [VIDEO]

Transfection is the process that introduces DNA or RNA into eukaryotic cells. It is a common and essential method to study and modulate gene regulation, which allows for a better understanding of gen...
Keywords: Gene expression, Genomics, Proteomics, Transfection

Transfection Efficiency and Optimization

The two main purposes of transfection are to produce recombinant proteins and to specifically enhance or inhibit gene expression in transfected cells. As such, transfection is a powerful analytical t...
Keywords: Gene expression, Transfection

Peer-Reviewed Papers
15

References

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