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T4GENE32-RO Roche

T4 Gene 32 Protein

Synonym: gene 32 protein, t4

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Properties

Related Categories Molecular Biology, PCR Reaction Components, PCR/Amplification More...
Quality Level   100
biological source   Escherichia coli (infected with phage T4amN134/amBL292/amE218)
form   solution
packaging   pkg of 100 μg (10972983001)
  pkg of 500 μg (10972991001)
mfr. no.   Roche
optimum pH   ~8.0
shipped in   dry ice
storage temp.   −20°C

Description

General description

T4 Gene 32 Protein is reported to improve the yield of long PCR products when 0.5 to 1.0 nmol of the protein is included in the reaction. T4 Gene 32 Protein is also reported to increase the yield of PCR products amplified from samples that contain humic acid. The inhibitory effect of humic acid is reduced by a factor of 7 when T4 Gene 32 Protein is included in the PCR (at a concentration of 2.5 μg/100 μl).
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.

Specificity

T4 gene 32 protein is a single-strand specific, helix-destabilizing protein encoded by gene 32 of the phage T4 genome. It is required for the replication of DNA and genetic recombination in Escherichia coli cells that are infected with T4 bacteriophage.

Application

T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:
• Optimization of PCR (See "Product Description" below.)
• Stimulation of in vitro DNA synthesis
• Stabilization of single-stranded regions of DNA or RNA
• Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
• Helping restriction enzyme digestions go to completion
• q-PCR

Quality

Absence of contaminants
T4 gene 32 protein is incubated with various nucleic acids that serve as potential nuclease substrates. Results are analyzed by gel electrophoresis. Based on these tests, none of the following are detectable in the preparation according to the current Quality Control procedures:
• nonspecific exo- and endonucleases
• single-strand DNases (nicking activities)
• single-strand-specific exonucleases
• RNases

Physical form

Protein solution, 1 - 10mg/ml, in storage buffer (20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 0.5mM DTT, 50% glycerol [v/v], pH approximately 8.0)

Preparation Note

Working concentration: 1 to 10 mg/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

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Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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