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TRYPSEQM-RO Roche

Trypsin Sequencing Grade, modified

from bovine pancreas

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Properties

Related Categories Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteolytic Enzymes, Proteolytic Enzymes and Substrates
Quality Level   100
form   lyophilized (salt-free)
packaging   pkg of 4 × 100 μg (11418033001)
  pkg of 4 × 25 μg (11418025001)
mfr. no.   Roche
optimum pH   8.0
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.

Inhibitors:
TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.

Specificity

Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.

Application

Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:
• Protein-structure elucidation
• Tryptic mapping
• Fingerprinting analysis
• Sequence analysis
• Translocation studies
• Protein identification

Quality

Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.

Preparation Note

Working concentration: 1/100 to 1/5 of the protein by weight
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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