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XTG360-RO Roche

X-tremeGENE 360 Transfection Reagent

Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines

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Properties

Related Categories Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology, Synthetic Biology,
grade   for molecular biology
form   liquid (aqueous solution)
packaging   pkg of 0.4 mL (08724105001)
  pkg of 1.0 mL (08724121001)
  pkg of 5 × 1.0 mL (08724156001)
mfr. no.   Roche
shipped in   wet ice
storage temp.   −20°C

Description

General description

X-tremeGENE 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE 360 delivers high efficiency transfection in more cell types with low cytotoxicity.

Application

X-tremeGENE 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.
• Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
• Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
• Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
• Suitable for transient and stable transfection.
• Functions well in the presence or absence of serum.

Features and Benefits

X-tremeGENE 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE 360 exhibits:
• Peak performance in a broad range of cell types
• Suitable for multiple applications
• Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

Quality

Each lot of X-tremeGENE 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
Keywords: Antibiotics, Diagnostic, Gene expression, Genetics, Transfection, Western blot

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA enco...
Keywords: Diagnostic, Gene knockdown, Transcription, Transfection

Introduction to Cell Transfection

Transfection involves the introduction of DNA or RNA into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques serve as an analytical tool tha...
Keywords: Apoptosis, Biological techniques, Drug discovery, Gene expression, Genetic, Precipitation, Transfection, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Reverse transfection of plasmid DNA

Automation is used for many applications to reduce variation caused by manual han­dling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown s...
Keywords: Cell culture, Transfection

Roche Transfection Database

X-tremeGENE™ Transfection Reagents from Roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells. Use the table below to find the right produ...
Keywords: Apoptosis, Cancer, Clinical, Cloning, DNA replication, Gene expression, Immunoprecipitation, In Situ hybridization, Individual protein Immunoprecipitation, Ion-exchange chromatography, Methylations, Microscopy, Nucleic acid hybridization, Polymerase chain reaction, Recombination, Transcription, Transfection, Western blot

Transfection Best Practices

Successful transfection of nucleic acids and proteins depends on numerous factors that range from reagent and cell type selection to protocol optimization. Ensure optimal quality in your experiment b...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Infrared spectroscopy, Ion-exchange chromatography, Transfection

X-tremeGENE™ Transfection Reagents General Recommendations

The ability to successfully transfect a cell varies significantly depending on the cell type, protocol, and the composition of the transfection reagent. X-tremeGENE™ transfection reagents are designe...
Keywords: Cancer, Cell culture, Cellular processes, Diagnostic, Gene expression, Gene knockdown, Microscopy, Transfection

X-tremeGENE™ siRNA Transfection Reagent: A Powerful Tool for Antisense Inhibition of miRNA in Human Cells with miRCURY Knockdown Probes

MicroRNAs (miRNAs) are an endogenous class of RNA molecules comprising 19-25 nucleotides that regulate the suitability or transflational efficiency of mRNAs. The mature miRNAs are processed from long...
Keywords: Apoptosis, Cancer, Cell proliferation, Diagnostic, Gene expression, In Situ hybridization, Nucleic acid hybridization, Transfection

Protocols

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol...
Keywords: Cell culture, Diagnostic, Gene expression, Microscopy, Recombination, Transduction, Transfection

Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ transfection reagents provide reliable transfection of diverse nucleic acids and CRISPR/Cas9 components, a critical step for your molecular and cellular research. Explore our list of the...
Keywords: Diagnostic, Gene expression, Transfection

X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

siRNA IN PRIMARY LUNG FIBROBLASTS TRANSFECTION Roche has no data for this application, but there is a report from a customer, briefly described below. Wild rat lung fibroblasts were successfully cotr...
Keywords: Gene expression, Growth factors, Microarray Analysis, Transfection, Western blot

Related Content

Cloning & Expression: Expression Vectors, Competent Cells, Transfection Reagents

The aim of molecular cloning is to insert the gene-of-interest (GOI) into a plasmid vector. This vector is then inserted into a cell that will express the protein encoded by the GOI. Once protein is ...
Keywords: Cell signaling, Cloning, Culture media, Gene expression, Genetics, Genomics, Molecular biology, Transfection

How Transfection Works [VIDEO]

Transfection is the process that introduces DNA or RNA into eukaryotic cells. It is a common and essential method to study and modulate gene regulation, which allows for a better understanding of gen...
Keywords: Gene expression, Genomics, Proteomics, Transfection

Roche Products and Biochemical Reagents

Whether working within a competitive funding landscape or tasked with hitting key business milestones towards financial deliverables, research scientists are also presented with more scientific infor...
Keywords: Cell culture, Genomics, Molecular biology, Polymerase chain reaction, Proteomics, Transfection

Transfection Efficiency and Optimization

The two main purposes of transfection are to produce recombinant proteins and to specifically enhance or inhibit gene expression in transfected cells. As such, transfection is a powerful analytical t...
Keywords: Gene expression, Transfection

X-tremeGENE™ Transfection Reagents Customer Testimony

The ability to transfect cells is often critical for researchers investigating a variety of diverse cellular processes in healthy and diseased tissues. Discover in the video below how Alan Alfano, a ...
Keywords: Cancer, Cellular processes, Diagnostic, Transfection

X-tremeGENE™ Transfection Reagents

With a joint commitment to enable your next discovery, we exclusively offer the complete portfolio of Roche X-tremeGENE™ transfection reagents. Whether you are working with common or difficult-to-tra...
Keywords: Cancer, Cell biology, Cell culture, Diagnostic, Molecular biology, Transfection

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