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XTG360-RO Roche

X-tremeGENE 360 Transfection Reagent

Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines



Related Categories Application: Polymer-based reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP into many cells, Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology,
grade   for molecular biology
form   liquid (aqueous solution)
packaging   pkg of 0.4 mL (08724105001)
  pkg of 1.0 mL (08724121001)
  pkg of 5 × 1.0 mL (08724156001)
mfr. no.   Roche
application(s)   transfection: suitable
shipped in   wet ice
storage temp.   −20°C


General description

X-tremeGENE 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE 360 delivers high efficiency transfection in more cell types with low cytotoxicity.


X-tremeGENE 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.
• Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
• Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
• Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
• Suitable for transient and stable transfection.
• Functions well in the presence or absence of serum.

Features and Benefits

X-tremeGENE 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE 360 exhibits:
• Peak performance in a broad range of cell types
• Suitable for multiple applications
• Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components


Each lot of X-tremeGENE 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
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Co-transfection of Plasmid DNA

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Introduction to Cell Transfection

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Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Reverse transfection of plasmid DNA

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Roche Transfection Database

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Transfection Best Practices

Successful transfection of nucleic acids and proteins depends on numerous factors that range from reagent and cell type selection to protocol optimization. Ensure optimal quality in your experiment b...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
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X-tremeGENE™ Transfection Reagents General Recommendations

The ability to successfully transfect a cell varies significantly depending on the cell type, protocol, and the composition of the transfection reagent. X-tremeGENE™ transfection reagents are designe...
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X-tremeGENE™ siRNA Transfection Reagent: A Powerful Tool for Antisense Inhibition of miRNA in Human Cells with miRCURY Knockdown Probes

MicroRNAs (miRNAs) are an endogenous class of RNA molecules comprising 19-25 nucleotides that regulate the suitability or transflational efficiency of mRNAs. The mature miRNAs are processed from long...
Keywords: Apoptosis, Cancer, Cell proliferation, Diagnostic, Gene expression, In Situ hybridization, Nucleic acid hybridization, Transfection


Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol...
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Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ transfection reagents provide reliable transfection of diverse nucleic acids and CRISPR/Cas9 components, a critical step for your molecular and cellular research. Explore our list of the...
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X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
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X-tremeGENE™ 9 DNA Transfection Reagent Protocol

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X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

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Related Content

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How Transfection Works [VIDEO]

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X-tremeGENE™ Transfection Reagents Customer Testimony

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