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XTG9-RO Roche

X-tremeGENE 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines



Related Categories Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology, Synthetic Biology,
grade   for molecular biology
form   liquid ((aqueous solution))
usage   1 mL (suitable for 165 transfections)
packaging   pkg of 0.4 mL (06365779001)
  pkg of 1.0 mL (06365787001)
  pkg of 5 × 1.0 mL (06365809001)
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C


Features and Benefits

• Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
• Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
• Avoid time-consuming optimization procedures in commonly used cell lines.

General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Other Notes

For life science research only. Not for use in diagnostic procedures.


Each lot of X-tremeGENE 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.


X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
• Expression of recombinant proteins for functional analysis.
• Physiological studies of metabolic pathways.
• Analysis of regulatory sequences using reporter gene assays.
• Gene expression assays.
• Cancer research studies.
• Target evaluation.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

UN1170 - class 3 - PG 2 - Ethanol, solution


Certificate of Analysis

Protocols & Articles


A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
Keywords: Antibiotics, Diagnostic, Gene expression, Genetics, Transfection, Western blot

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA enco...
Keywords: Diagnostic, Gene knockdown, Transcription, Transfection

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Reverse transfection of plasmid DNA

Automation is used for many applications to reduce variation caused by manual han­dling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown s...
Keywords: Cell culture, Transfection

Roche Transfection Database

Roche X-tremeGENE™ Transfection Reagents have been used to transfect hundreds of different cell lines, primary cells & stem cells. To find the right product for your cells, use the Selection Guide be...
Keywords: Apoptosis, Cancer, Clinical, Cloning, DNA replication, Gene expression, Immunoprecipitation, In Situ hybridization, Individual protein Immunoprecipitation, Ion-exchange chromatography, Methylations, Microscopy, Nucleic acid hybridization, Polymerase chain reaction, Recombination, Transcription, Transfection, Western blot

Transfection Best Practices

The work of scientific research is an art which requires good attention to detail.  The quality of transfection is highest when these commonly used best practices and notes on optimization are employ...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

X-tremeGENE™ General Recommendations

Note: The above data was produced using either a GFP-encoding pcDNA3.1 plasmid or a luciferase-encoding pCI plasmid, both with the cytomegalovirus (CMV) promoter. These recommendations are guidelines...
Keywords: Cancer, Cellular processes, Diagnostic, Gene expression, Gene knockdown, Transfection


Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagent

Plate CHO-K1 cells at a density of 1.5 X 104 cells/well. Plate cells in a volume of 100 μl complete growth medium per well in a 96-well plate 18 – 24 hours before transfection (65 – 75% confluency). ...
Keywords: Diagnostic, Gene expression, Transfection

X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

Peer-Reviewed Papers


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