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XTG9-RO Roche

X-tremeGENE 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines



Related Categories Application: For commonly used cells, Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology,
Quality Level   100
grade   for molecular biology
form   liquid (aqueous solution)
usage    mL (suitable for 165 transfections)
packaging   pkg of 0.4 mL (06365779001)
  pkg of 1.0 mL (06365787001)
  pkg of 5 × 1.0 mL (06365809001)
mfr. no.   Roche
application(s)   transfection: suitable
shipped in   wet ice
storage temp.   2-8°C


General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.


X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
• Expression of recombinant proteins for functional analysis.
• Physiological studies of metabolic pathways.
• Analysis of regulatory sequences using reporter gene assays.
• Gene expression assays.
• Cancer research studies.
• Target evaluation.

Features and Benefits

• Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
• Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
• Avoid time-consuming optimization procedures in commonly used cell lines.


Each lot of X-tremeGENE 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

UN1170 - class 3 - PG 2 - Ethanol, solution


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


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Introduction to Cell Transfection

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Molecular Biology Handbook

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The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
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Automation is used for many applications to reduce variation caused by manual han­dling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown s...
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Roche Transfection Database

X-tremeGENE™ Transfection Reagents from Roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells. Use the table below to find the right produ...
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Transfection Best Practices

Successful transfection of nucleic acids and proteins depends on numerous factors that range from reagent and cell type selection to protocol optimization. Ensure optimal quality in your experiment b...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Infrared spectroscopy, Ion-exchange chromatography, Transfection

X-tremeGENE™ Transfection Reagents General Recommendations

The ability to successfully transfect a cell varies significantly depending on the cell type, protocol, and the composition of the transfection reagent. X-tremeGENE™ transfection reagents are designe...
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Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ transfection reagents provide reliable transfection of diverse nucleic acids and CRISPR/Cas9 components, a critical step for your molecular and cellular research. Explore our list of the...
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X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
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X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
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Related Content

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Peer-Reviewed Papers


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