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X-tremeGENE HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines



Related Categories Application: For hard-to-transfect cells, Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology,
Quality Level   100
grade   for molecular biology
form   liquid (aqueous solution)
usage    mL (suitable for 165 transfections)
packaging   pkg of 0.4 mL (06366244001)
  pkg of 1.0 mL (06366236001)
  pkg of 5 × 1 mL (06366546001)
mfr. no.   Roche
application(s)   transfection: suitable
shipped in   wet ice
storage temp.   −20°C


General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

• Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
• Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
• Generate physiologically relevant data using a reagent with low cytotoxic effects.
• Increase experimental throughput and enable target evaluation using a simple and consistent protocol.


Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

UN1170 - class 3 - PG 2 - Ethanol, solution


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
Keywords: Antibiotics, Diagnostic, Gene expression, Genetics, Transfection, Western blot

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA enco...
Keywords: Diagnostic, Gene knockdown, Transcription, Transfection

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques serve as an analytical too...
Keywords: Apoptosis, Biological techniques, Drug discovery, Gene expression, Genetic, Precipitation, Transduction, Transfection, transformation

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Reverse transfection of plasmid DNA

Automation is used for many applications to reduce variation caused by manual han­dling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown s...
Keywords: Cell culture, Transfection

Roche Transfection Database

X-tremeGENE™ Transfection Reagents from Roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells. Use the table below to find the right produ...
Keywords: Apoptosis, Cancer, Clinical, Cloning, DNA replication, Gene expression, Immunoprecipitation, In Situ hybridization, Individual protein Immunoprecipitation, Ion-exchange chromatography, Methylations, Microscopy, Nucleic acid hybridization, Polymerase chain reaction, Recombination, Transcription, Transfection, Western blot

Transfection Best Practices

Successful transfection of nucleic acids and proteins depends on numerous factors that range from reagent and cell type selection to protocol optimization. Ensure optimal quality in your experiment b...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Infrared spectroscopy, Ion-exchange chromatography, Transfection


Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol...
Keywords: Cell culture, Diagnostic, Gene expression, Microscopy, Recombination, Transduction, Transfection

Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ transfection reagents provide reliable transfection of diverse nucleic acids and CRISPR/Cas9 components, a critical step for your molecular and cellular research. Explore our list of the...
Keywords: Diagnostic, Gene expression, Transfection

X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

Related Content

How Transfection Works [VIDEO]

Transfection is the process that introduces DNA or RNA into eukaryotic cells. It is a common and essential method to study and modulate gene regulation, which allows for a better understanding of gen...
Keywords: Gene expression, Genomics, Proteomics, Transfection

Transfection Efficiency and Optimization

The two main purposes of transfection are to produce recombinant proteins and to specifically enhance or inhibit gene expression in transfected cells. As such, transfection is a powerful analytical t...
Keywords: Gene expression, Transfection

X-tremeGENE™ Transfection Reagents Customer Testimony

The ability to transfect cells is often critical for researchers investigating a variety of diverse cellular processes in healthy and diseased tissues. Discover in the video below how Alan Alfano, a ...
Keywords: Cancer, Cellular processes, Diagnostic, Transfection

Peer-Reviewed Papers


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