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XTGHP-RO Roche

X-tremeGENE HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

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Properties

Related Categories Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology, Synthetic Biology,
grade   for molecular biology
form   liquid ((aqueous solution))
usage   1 mL (suitable for 165 transfections)
packaging   pkg of 0.4 mL (06366244001)
  pkg of 1.0 mL (06366236001)
  pkg of 5 × 1 mL (06366546001)
mfr. no.   Roche
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

• Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
• Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
• Generate physiologically relevant data using a reagent with low cytotoxic effects.
• Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

General description

Download the CofA, SDS and IFU specific to your product lot and format

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Quality

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
RIDADR 
UN1170 - class 3 - PG 2 - Ethanol, solution

Documents

Certificate of Analysis

Protocols & Articles

Articles

A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
Keywords: Antibiotics, Diagnostic, Gene expression, Genetics, Transfection, Western blot

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA enco...
Keywords: Diagnostic, Gene knockdown, Transcription, Transfection

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Reverse transfection of plasmid DNA

Automation is used for many applications to reduce variation caused by manual han­dling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown s...
Keywords: Cell culture, Transfection

Roche Transfection Database

Roche X-tremeGENE™ Transfection Reagents have been used to transfect hundreds of different cell lines, primary cells & stem cells. To find the right product for your cells, use the Selection Guide be...
Keywords: Apoptosis, Cancer, Clinical, Cloning, DNA replication, Gene expression, Immunoprecipitation, In Situ hybridization, Individual protein Immunoprecipitation, Ion-exchange chromatography, Methylations, Microscopy, Nucleic acid hybridization, Polymerase chain reaction, Recombination, Transcription, Transfection, Western blot

Transfection Best Practices

The work of scientific research is an art which requires good attention to detail.  The quality of transfection is highest when these commonly used best practices and notes on optimization are employ...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Protocols

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types. During virus production, low titers and toxicity can be challenging. This easy-to-use protocol...
Keywords: Cell culture, Diagnostic, Gene expression, Microscopy, Recombination, Transduction, Transfection

Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagent

Plate CHO-K1 cells at a density of 1.5 X 104 cells/well. Plate cells in a volume of 100 μl complete growth medium per well in a 96-well plate 18 – 24 hours before transfection (65 – 75% confluency). ...
Keywords: Diagnostic, Gene expression, Transfection

X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

Related Content

X-tremeGENE™ Customer Testimony

X-tremeGENE is a trademark of Roche. For life science research only. Not for use in diagnostic procedures.
Keywords: Diagnostic, Transfection

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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