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05-2605 Sigma-Aldrich

CHAPS

≥97.0%

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

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Properties

Related Categories Biochemicals and Reagents, Core Bioreagents, Detergents, Detergents A to M, Research Essentials,
description   zwitterionic
assay   ≥97.0%
mol wt   micellar avg mol wt 6150
availability   available only in Japan
aggregation number   10
application(s)   dialysis: suitable
  electrophoresis: suitable
  protein quantification: suitable
CMC   6 mM (20-25°C)
transition temp   cloud point >100 °C
SMILES string   C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI   1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
InChI key   UMCMPZBLKLEWAF-RFCNGIAKSA-N

Description

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Suitability

for biological purposes

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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