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00185 Millipore

Acetamide Nutrient Broth

suitable for microbiology, NutriSelect® Basic



Related Categories A - M, All Liquid Media/Broths, Biochemical Identification Media, A to Z, Identification Tests & Reagents, Media by Application,
Quality Level   100
form   powder
shelf life   limited shelf life, expiry date on the label
composition   acetamide, 2.0 g/L
  dipotassium hydrogen phosphate, 0.2 g/L
  ferrous sulfate, 0.0005 g/L
  magnesium sulfate, 0.158 g/L
  sodium chloride, 0.2 g/L
  sodium molybdate, 0.005 g/L
mfr. no.   NutriSelect® Basic
application(s)   bioburden testing: suitable
  microbiology: suitable (microbial identification)
  microbiology: suitable
  pathogen testing: suitable
  water monitoring: suitable
final pH   7.0±0.2 (25 °C)
Featured Industry   Environmental
Food and Beverages
storage temp.   room temp
suitability   selective by defined nutrients for Pseudomonas spp.



Synthetic medium to detect microbial utilization of acetamide. Corresponds to the suggestions of the "Deutsches Institut für Normung (DIN)" and the test procedures set out in §35 LMBG for the examination of water for Pseudomonas aeruginosa.

Preparation Note

Dissolve 2.56 g in 950 ml distilled water. Check the pH and if necessary adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before.going to the isolation medium.To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent (Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.

Other Notes

Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas; Comparative studies on the enrichment of P. aeruginosa

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Peer-Reviewed Papers


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