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01696 Sigma-Aldrich

Acrylamide

for Northern and Southern blotting, powder blend

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1

  • Linear Formula CH2=CHCONH2

  • Molecular Weight 71.08

  •  Beilstein/REAXYS Number 605349

  •  EC Number 201-173-7

  •  MDL number MFCD00008032

  •  eCl@ss 39031205

  •  PubChem Substance ID 329747346

  •  NACRES NA.25

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Properties

Quality Level   200
grade   for molecular biology
vapor density   2.45 (vs air)
vapor pressure   0.03 mmHg ( 40 °C)
product line   BioReagent
assay   ≥99.5% (GC)
form   powder blend
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
bp   125 °C/25 mmHg (lit.)
mp   82-86 °C (lit.)
solubility   H2O: 0.25 g/mL at 20 °C, clear, colorless
storage temp.   2-8°C
SMILES string   NC(=O)C=C
InChI   1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
InChI key   HRPVXLWXLXDGHG-UHFFFAOYSA-N

Description

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Application

Suitable for making polyacrylamide gels according to established recipes.

Caution

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

Safety & Documentation
Protocols & Articles

Articles

Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Introduction to Markers and Ladders

We offer a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, PAGE, Polymerase chain reaction, Proteomics

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Peer-Reviewed Papers
15

References

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