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03450 Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

purum, ≥98.0% (AT)

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride, EDC, WSC hydrochloride

  • CAS Number 25952-53-8

  • Empirical Formula (Hill Notation) C8H17N3 · HCl

  • Molecular Weight 191.70

  •  Beilstein/REAXYS Number 5764110

  •  EC Number 247-361-2

  •  MDL number MFCD00012503

  •  PubChem Substance ID 329747708

  •  NACRES NA.31

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Properties

Related Categories Crosslinking, Heterobifunctional Cross-Linking Reagents, Molecular Biology, Protein Modification, Protein Structural Analysis,
Quality Level   200
grade   purum
assay   ≥98.0% (AT)
mp   110-115 °C (lit.)
  110-115 °C
solubility   H2O: soluble 1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow
storage temp.   −20°C
SMILES string   Cl.CCN=C=NCCCN(C)C
InChI   1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key   FPQQSJJWHUJYPU-UHFFFAOYSA-N

Description

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used modify the cell surface of Escherichia coli to covalently couple substances. It has also been used as an activator to modify microfluidic chips to capture Escherichia coli.

Biochem/physiol Actions

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Safety & Documentation

Safety Information

Signal word 
Danger
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
RTECS 
FF2200000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Carbodiimides

Carbodiimide-mediated peptide coupling remains to the most frequently used technique. As a major advantage, carbodiimides do not require prior activation of the carboxylic acid. Dicyclohexylcarbodiim...
Matthias Junkers
Aldrich ChemFiles 2007, 7.2, 5.
Keywords: Cyclizations, Peptide synthesis, Solid phase peptide synthesis

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The biochemicals you use in your experiments directly impact the quality and reproducibility of your results. Biochemicals from us are world class products, competitively priced, and backed by superi...
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Peer-Reviewed Papers
15

References

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