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03553 Supelco

Griess’ reagent for nitrite

for microscopy

Synonym: Griess-Ilosvays Reagent for nitrate, Lunge’s Reagent



Related Categories Analytical Reagents, Analytical/Chromatography, Application Index, Biochemicals and Reagents, Cell Signaling Enzymes,
grade   for microscopy
impurities   2-naphthylamine
  acetic acid
refractive index   n20/D 1.353
density   1.037 g/mL at 20 °C
Featured Industry   Food and Beverages
General Analytical


General description

The Griess reagent is used for the quantitative analysis of nitrite ions. Present nitrite (as well as nitrate) ions will form a pink diazo dye by diazonium coupling reaction with N-(1-Naphthyl)ethylenediamine. The concentration of nitrite (or nitrate) ions is proportional to the color intensity of the resulting red solution.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN 2790B 8 / PGII
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
When using Product 03553, Griess reagent for nitrite, what product should I use to make a nitrite standard curve?
Sodium nitrite (Product No. S2252 or 237213) can be used to make a standard curve. A typical standard curve would cover the range of 0-100 microMolar nitrite.
Will phenol red interfere with the Griess reaction, Product 03553, Griess reagent for nitrite?
No. Phenol red (which is a pH indicator present in some cell culture media) will not interfere with the Griess reaction.
Is there an enzymatic method to convert nitrate to nitrite?
A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996).For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).
What is the difference between Product G4410 Griess reagent (modified), and Product 03553, Griess reagent?
There are many formulations of the Griess Reagent.The formulations for these products are similar; both are proprietary.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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