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03677 Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

BioUltra, for molecular biology, ≥99.0% (T)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

  • CAS Number 6381-92-6

  • Empirical Formula (Hill Notation) C10H14N2Na2O8 · 2H2O

  • Molecular Weight 372.24

  •  Beilstein/REAXYS Number 3900609

  •  EC Number 205-358-3

  •  PubChem Substance ID 24845544

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
Quality Level   200
grade   for molecular biology
product line   BioUltra
assay   ≥99.0% (T)
form   powder or crystals
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   4.0-5.5 (25 °C, 0.1 M in H2O)
mp   248 °C (dec.) (lit.)
solubility   H2O: 0.1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.145
  λ: 280 nm Amax: 0.025
SMILES string   O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI   1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
InChI key   OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

Application

Ethylenediaminetetraacetic acid disodium salt dehydrate can be used to study molecular biology, biochemicals, solutions and reagents, biological buffers, DNA and RNA purification, inorganic salts, essential chemicals and chelators. Ethylenediaminetetraacetic acid disodium salt dehydrate has been used in a study to advance monitoring of illegal thyreostatic drugs in livestock. Ethylenediaminetetraacetic acid disodium salt dehydrate has also been used in a study for the determination of 232Th in seawater by ICP-MS after preconcentration and separation using a chelating resin.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Other Notes

Running-buffer component for non-denaturing or urea-containing denaturing polyacrylamide gels in nucleic acid electrophoresis

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory Tract
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
RTECS 
AH4410000
Flash Point(F) 
No data available
Flash Point(C) 
No data available

Documents

Certificate of Analysis (COA)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

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Peer-Reviewed Papers
15

References

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